Salmonella enterica prevalence in Leatherback Sea Turtles (Dermochelys Coriacea) in St. Kitts, West Indies

Clayton S. Dutton, Floyd Revan, Chengming Wang, Chuanling Xu, Terry M. Norton, Kimberly M. Stewart, Bernhard Kaltenboeck, Esteban Soto Martinez

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Salmonella spp. are gram-negative bacteria capable of causing diseases in a wide range of aquatic and terrestrial animals, including humans. Sea and terrestrial turtles have been recognized as carriers of this zoonotic pathogen. In this project, conventional and molecular diagnostic methods were combined to investigate the prevalence of Salmonella enterica in leatherback sea turtles (Dermochelys coriacea) that used the island of St. Kitts, West Indies as a nesting ground during 2011 (n = 21). Isolates obtained from selective media were screened and colonies suspected of being Salmonella spp. were confirmed by fluorescence resonance energy transfer polymerase chain reaction. The prevalence of S. enterica within this sample population during this period was found to be 14.2%. Moreover, due to the increasing risk of antibiotic resistance in enteric bacteria, antimicrobial susceptibility was investigated in all recovered Salmonella spp. isolates utilizing the broth microdilution method. All isolates were susceptible to the lowest concentration of kanamycin, gentamicin, ciprofloxacin, enrofloxacin, nalidixic acid, and trimethoprim/sulfamethoxazole tested. Further research should be pursued to understand the interaction of this bacterial pathogen with the environment, host, and other microbial communities, and to further develop faster, more sensitive, and more specific diagnostic methods.

Original languageEnglish (US)
Pages (from-to)765-768
Number of pages4
JournalJournal of Zoo and Wildlife Medicine
Volume44
Issue number3
DOIs
StatePublished - Sep 2013
Externally publishedYes

Fingerprint

Dermochelys coriacea
West Indies
Turtles
Salmonella enterica
sea turtles
Salmonella
diagnostic techniques
Nalidixic Acid
Fluorescence Resonance Energy Transfer
Kanamycin
sulfamethoxazole
nalidixic acid
Molecular Pathology
pathogens
trimethoprim
enrofloxacin
ciprofloxacin
selective media
Sulfamethoxazole Drug Combination Trimethoprim
Zoonoses

Keywords

  • Leatherback sea turtles
  • PCR
  • Salmonella
  • St. Kitts
  • Zoonotic

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Salmonella enterica prevalence in Leatherback Sea Turtles (Dermochelys Coriacea) in St. Kitts, West Indies. / Dutton, Clayton S.; Revan, Floyd; Wang, Chengming; Xu, Chuanling; Norton, Terry M.; Stewart, Kimberly M.; Kaltenboeck, Bernhard; Soto Martinez, Esteban.

In: Journal of Zoo and Wildlife Medicine, Vol. 44, No. 3, 09.2013, p. 765-768.

Research output: Contribution to journalArticle

Dutton, Clayton S. ; Revan, Floyd ; Wang, Chengming ; Xu, Chuanling ; Norton, Terry M. ; Stewart, Kimberly M. ; Kaltenboeck, Bernhard ; Soto Martinez, Esteban. / Salmonella enterica prevalence in Leatherback Sea Turtles (Dermochelys Coriacea) in St. Kitts, West Indies. In: Journal of Zoo and Wildlife Medicine. 2013 ; Vol. 44, No. 3. pp. 765-768.
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