Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains

Ming Wen Li, Kristy L. Kinchen, Jadine M. Vallelunga, Diana L. Young, Kaleb D K Wright, Lisa N. Gorano, Katherine Wasson, Kevin C K Lloyd

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the present report we studied the safety, efficacy and efficiency of using an infrared laser to facilitate IVF by assessing fertilization, development and birth rates after laser-zona drilling (LZD) in 30 subfertile genetically modified (GM) mouse lines. We determined that LZD increased the fertilization rate four to ten times that of regular IVF, thus facilitating the derivation of 26 of 30 (86.7%) GM mouse lines. Cryopreserved two-cell stage embryos derived by LZD-assisted IVF were recovered and developed to blastocysts in vitro at the same rate as frozen-thawed embryos derived by regular IVF. Surprisingly after surgical transfer to pseudopregnant recipients the birth rate of embryos derived by LZD-assisted IVF was significantly lower than that of embryos derived by regular IVF. However this result could be completely mitigated by the addition of 0.25 M sucrose to the culture medium during LZD which caused the oocyte to shrink in volume relative to the perivitelline space. By increasing the distance from the laser target site on the zona pellucida, we hypothesize that the hyperosmotic effect of sucrose reduced the potential for laser-induced cytotoxic thermal damage to the underlying oocytes. With appropriate preparation and cautious application, our results indicate that LZD-assisted IVF is a safe, efficacious and efficient assisted reproductive technology for deriving mutant mouse lines with male factor infertility and subfertility caused by sperm-zona penetration defects.

Original languageEnglish (US)
Pages (from-to)245-254
Number of pages10
JournalReproduction
Volume145
Issue number3
DOIs
StatePublished - Mar 2013

Fingerprint

Mutant Strains Mice
Herpes Zoster
Lasers
Safety
Embryonic Structures
Birth Rate
Male Infertility
Fertilization
Oocytes
Sucrose
Sperm-Ovum Interactions
Zona Pellucida
Assisted Reproductive Techniques
Blastocyst
Culture Media
Hot Temperature

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Li, M. W., Kinchen, K. L., Vallelunga, J. M., Young, D. L., Wright, K. D. K., Gorano, L. N., ... Lloyd, K. C. K. (2013). Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains. Reproduction, 145(3), 245-254. https://doi.org/10.1530/REP-12-0477

Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains. / Li, Ming Wen; Kinchen, Kristy L.; Vallelunga, Jadine M.; Young, Diana L.; Wright, Kaleb D K; Gorano, Lisa N.; Wasson, Katherine; Lloyd, Kevin C K.

In: Reproduction, Vol. 145, No. 3, 03.2013, p. 245-254.

Research output: Contribution to journalArticle

Li, MW, Kinchen, KL, Vallelunga, JM, Young, DL, Wright, KDK, Gorano, LN, Wasson, K & Lloyd, KCK 2013, 'Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains', Reproduction, vol. 145, no. 3, pp. 245-254. https://doi.org/10.1530/REP-12-0477
Li MW, Kinchen KL, Vallelunga JM, Young DL, Wright KDK, Gorano LN et al. Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains. Reproduction. 2013 Mar;145(3):245-254. https://doi.org/10.1530/REP-12-0477
Li, Ming Wen ; Kinchen, Kristy L. ; Vallelunga, Jadine M. ; Young, Diana L. ; Wright, Kaleb D K ; Gorano, Lisa N. ; Wasson, Katherine ; Lloyd, Kevin C K. / Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains. In: Reproduction. 2013 ; Vol. 145, No. 3. pp. 245-254.
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