Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies

Heidi J. Chial, Michael P. Rout, Thomas H. Giddings, Mark Winey

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

We report a novel connection between nuclear pore complexes (NPCs) and spindle pole bodies (SPBs) revealed by our studies of the Saccharomyces cerevisiae NDC1 gene. Although both NPCs and SPBs are embedded in the nuclear envelope (NE) in yeast, their known functions are quite distinct. Previous work demonstrated that NDC1 function is required for proper SPB duplication (Winey, M., M.A. Hoyt, C. Chan, L. Goetsch, D. Botstein, and B. Byers. 1993. J. Cell Biol. 122:743-751). Here, we show that Ndc1p is a membrane protein of the NE that localizes to both NPCs and SPBs. Indirect immunofluorescence microscopy shows that Ndc1p displays punctate, nuclear peripheral localization that colocalizes with a known NPC component, Nup49p. Additionally, distinct spots of Ndclp localization colocalize with a known SPB component, Spc42p. Immunoelectron microscopy shows that Ndclp localizes to the regions of NPCs and SPBs that interact with the NE. The NPCs in ndc1- 1 mutant cells appear to function normally at the nonpermissive temperature. Finally, we have found that a deletion of POM152, which encodes an abundant but nonessential nucleoporin, suppresses the SPB duplication defect associated with a mutation in the NDC1 gene. We show that Ndclp is a shared component of NPCs and SPBs and propose a shared function in the assembly of these organelles into the NE.

Original languageEnglish (US)
Pages (from-to)1789-1800
Number of pages12
JournalJournal of Cell Biology
Volume143
Issue number7
DOIs
StatePublished - Dec 28 1998
Externally publishedYes

Fingerprint

Spindle Pole Bodies
Nuclear Pore
Saccharomyces cerevisiae
Nuclear Envelope
Nuclear Pore Complex Proteins
Immunoelectron Microscopy
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Organelles
Genes
Membrane Proteins
Yeasts
Mutation

Keywords

  • Ndc1p
  • Nuclear pore complex
  • Pom152p
  • Spindle pole body
  • Yeast

ASJC Scopus subject areas

  • Cell Biology

Cite this

Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies. / Chial, Heidi J.; Rout, Michael P.; Giddings, Thomas H.; Winey, Mark.

In: Journal of Cell Biology, Vol. 143, No. 7, 28.12.1998, p. 1789-1800.

Research output: Contribution to journalArticle

Chial, Heidi J. ; Rout, Michael P. ; Giddings, Thomas H. ; Winey, Mark. / Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies. In: Journal of Cell Biology. 1998 ; Vol. 143, No. 7. pp. 1789-1800.
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