Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope

María De La Cruz Muñoz-Centeno, Susan McBratney, Antonio Monterrosa, Breck Byers, Carl Mann, Mark Winey

Research output: Contribution to journalArticle

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Abstract

The MPS2 (monopolar spindle two) gene is one of several genes required for the proper execution of spindle pole body (SPB) duplication in the budding yeast Saccharomyces cerevisiae (Winey et al., 1991). We report here that the MPS2 gene encodes an essential 44-kDa protein with two putative coiled-coil regions and a hydrophobic sequence. Although MPS2 is required for normal mitotic growth, some null strains can survive; these survivors exhibit slow growth and abnormal ploidy. The MPS2 protein was tagged with nine copies of the myc epitope, and biochemical fractionation experiments show that it is an integral membrane protein. Visualization of a green fluorescent protein (GFP) Mps2p fusion protein in living cells and indirect immunofluorescence microscopy of 9xmyc-Mps2p revealed a perinuclear localization with one or two brighter foci of staining corresponding to the SPB. Additionally, immunoelectron microscopy shows that GFP-Mps2p localizes to the SPB. Our analysis suggests that Mps2p is required as a component of the SPB for insertion of the nascent SPB into the nuclear envelope.

Original languageEnglish (US)
Pages (from-to)2393-2406
Number of pages14
JournalMolecular Biology of the Cell
Volume10
Issue number7
StatePublished - Jul 1 1999
Externally publishedYes

Fingerprint

Spindle Pole Bodies
Nuclear Envelope
Saccharomyces cerevisiae
Membrane Proteins
Green Fluorescent Proteins
Proteins
Saccharomycetales
Immunoelectron Microscopy
Ploidies
Essential Genes
Indirect Fluorescent Antibody Technique
Growth
Fluorescence Microscopy
Genes
Epitopes
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

De La Cruz Muñoz-Centeno, M., McBratney, S., Monterrosa, A., Byers, B., Mann, C., & Winey, M. (1999). Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. Molecular Biology of the Cell, 10(7), 2393-2406.

Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. / De La Cruz Muñoz-Centeno, María; McBratney, Susan; Monterrosa, Antonio; Byers, Breck; Mann, Carl; Winey, Mark.

In: Molecular Biology of the Cell, Vol. 10, No. 7, 01.07.1999, p. 2393-2406.

Research output: Contribution to journalArticle

De La Cruz Muñoz-Centeno, M, McBratney, S, Monterrosa, A, Byers, B, Mann, C & Winey, M 1999, 'Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope', Molecular Biology of the Cell, vol. 10, no. 7, pp. 2393-2406.
De La Cruz Muñoz-Centeno M, McBratney S, Monterrosa A, Byers B, Mann C, Winey M. Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. Molecular Biology of the Cell. 1999 Jul 1;10(7):2393-2406.
De La Cruz Muñoz-Centeno, María ; McBratney, Susan ; Monterrosa, Antonio ; Byers, Breck ; Mann, Carl ; Winey, Mark. / Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. In: Molecular Biology of the Cell. 1999 ; Vol. 10, No. 7. pp. 2393-2406.
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