Saccharomyces cerevisiae cells lacking the homologous pairing protein p175SEP1 arrest at pachytene during meiotic prophase

Jürg Bähler, Gerrit Hagens, Gudrun Holzinger, Harry Scherthan, Wolf Dietrich Heyer

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1 enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At the arrest point, the cells remain largely viable and are cytologically characterized by the duplicated but unseparated spindle pole bodies of equal size and by the persistence of the synaptonemal complex, a cytological marker for pachytene. In addition, fluorescence in situ hybridization revealed that in arrested mutant cells maximal chromatin condensation and normal homolog pairing is achieved, typical for pachytene in wild type. A hallmark of meiosis is the high level of homologous recombination, which was analyzed both genetically and physically. Formation and processing of the double-strand break intermediate in meiotic recombination is achieved prior to arrest. Physical intragenic (conversion) and intergenic (crossover) products are formed just prior to, or directly at, the arrest point. Structural deficits in synaptonemal complex morphology, failure to separate spindle pole bodies, and/or defects in prophase DNA metabolism might be responsible for triggering the observed arrest. The pachytene arrest in sep1 cells is likely to be regulatory, but is clearly different from the RAD9 checkpoint in meiotic prophase, which occurs prior to the pachytene stage.

Original languageEnglish (US)
Pages (from-to)129-141
Number of pages13
JournalChromosoma
Volume103
Issue number2
DOIs
StatePublished - Apr 1994
Externally publishedYes

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Prophase
Spindle Pole Bodies
Synaptonemal Complex
Saccharomyces cerevisiae
Meiosis
Pachytene Stage
Null Lymphocytes
Proteins
Homologous Recombination
Body Size
Fluorescence In Situ Hybridization
Genetic Recombination
Chromatin
Mutation
DNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Saccharomyces cerevisiae cells lacking the homologous pairing protein p175SEP1 arrest at pachytene during meiotic prophase. / Bähler, Jürg; Hagens, Gerrit; Holzinger, Gudrun; Scherthan, Harry; Heyer, Wolf Dietrich.

In: Chromosoma, Vol. 103, No. 2, 04.1994, p. 129-141.

Research output: Contribution to journalArticle

Bähler, Jürg ; Hagens, Gerrit ; Holzinger, Gudrun ; Scherthan, Harry ; Heyer, Wolf Dietrich. / Saccharomyces cerevisiae cells lacking the homologous pairing protein p175SEP1 arrest at pachytene during meiotic prophase. In: Chromosoma. 1994 ; Vol. 103, No. 2. pp. 129-141.
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