RyR1-mediated Ca2+ leak and Ca2+ entry determine resting intracellular Ca2+ in skeletal myotubes

José M. Eltit, Tianzhong Yang, Hongli Li, Tadeusz F. Molinski, Isaac N Pessah, Paul D. Allen, José R. Lopez

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The control of resting free Ca2+ in skeletal muscle is thought to be a balance of channels, pumps exchangers in both the sarcolemma and sarcoplasmic reticulum. We explored these mechanisms using pharmacologic and molecular perturbations of genetically engineered (dyspedic) muscle cells that constitutively lack expression of the skeletal muscle sarcoplasmic reticulum Ca2+ release channels, RyR1 and RyR3. We demonstrate here that expression of RyR1 is responsible for more than half of total resting Ca 2+ concentration ([Ca2+]rest) measured in wild type cells. The elevated [Ca2+]rest in RyR1-expressing cells is not a result of active gating of the RyR1 channel but instead is accounted for by the RyR1 ryanodine-insensitive Ca2+ leak conformation. In addition, we demonstrate that basal sarcolemmal Ca2+ influx is also governed by RyR1 expression and contributes in the regulation of [Ca2+]rest in skeletal myotubes.

Original languageEnglish (US)
Pages (from-to)13781-13787
Number of pages7
JournalJournal of Biological Chemistry
Volume285
Issue number18
DOIs
StatePublished - Apr 30 2010

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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