RYR1 and RYR3 have different roles in the assembly of calcium release units of skeletal muscle

Feliciano Protasi, Hiroaki Takekura, Yaming Wang, S. R. Wayne Chen, Gerhard Meissner, Paul D. Allen, Clara Franzini-Armstrong

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Calcium release units (CRUs) are junctions between the sarcoplasmic reticulum (SR) and exterior membranes that mediates excitation contraction (e-c) coupling in muscle cells. In skeletal muscle CRUs contain two isoforms of the sarcoplasmic reticulum Ca2+ release channel: ryanodine receptors type 1 and type 3 (RyR1 and RyR3). 1B5s are a mouse skeletal muscle cell line that carries a null mutation for RyR1 and does not express either RyR1 or RyR3. These cells develop dyspedic SR/exterior membrane junctions (i.e., dyspedic calcium release units, dCRUs) that contain dihydropyridine receptors (DHPRs) and triadin, two essential components of CRUs, but no RyRs (or feet). Lack of RyRs in turn affects the disposition of DHPRs, which is normally dictated by a linkage to RyR subunits. In the dCRUs of 1B5 cells, DHPRs are neither grouped into tetrads nor aligned in two orthogonal directions. We have explored the structural role of RyR3 in the assembly of CRUs in 1B5 cells independently expressing either RyR1 or RyR3. Either isoform colocalizes with DHPRs and triadin at the cell periphery. Electron microscopy shows that expression of either isoform results in CRUs containing arrays of feet, indicating the ability of both isoforms to be targeted to dCRUs and to assemble in ordered arrays in the absence of the other. However, a significant difference between RyR1- and RyR3-rescued junctions is revealed by freeze fracture. While cells transfected with RyR1 show restoration of DHPR tetrads and DHPR orthogonal alignment indicative of a link to RyRs, those transfected with RyR3 do not. This indicates that RyR3 fails to link to DHPRs in a specific manner. This morphological evidence supports the hypothesis that activation of RyR3 in skeletal muscle cells must be indirect and provides the basis for failure of e-c coupling in muscle cells containing RyR3 but lacking RyR1 (see the accompanying report, Fessenden et al., 2000).

Original languageEnglish (US)
Pages (from-to)2494-2508
Number of pages15
JournalBiophysical Journal
Volume79
Issue number5
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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    Protasi, F., Takekura, H., Wang, Y., Wayne Chen, S. R., Meissner, G., Allen, P. D., & Franzini-Armstrong, C. (2000). RYR1 and RYR3 have different roles in the assembly of calcium release units of skeletal muscle. Biophysical Journal, 79(5), 2494-2508.