Ryanodine receptor type III (Ry3R) identification in mouse parotid acini. Properties and modulation of [3H]ryanodine-binding sites

Dennis H. DiJulio, Eileen L. Watson, Isaac N Pessah, Kerry L. Jacobson, Sabrina M. Ott, Edmond D. Buck, Jean C. Singh

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Abstract

Immunoblot analysis and [3H]ryanodine binding were used to characterize and identify ryanodine receptors (RyRs) in nonexcitable mouse parotid acini. Western analysis revealed ryanodine receptor type III (Ry3R) to be the only detectable isoform in parotid microsomal membranes. Binding of [3H]ryanodine to microsomal fractions was dependent on Ca2+, salt, pH, and temperature. At 23 °C, and in the presence of 0.5 M KCl and 100 μM Ca2+, [3H]ryanodine bound specifically to membranes with high affinity (K(d) = 6 nM); maximum binding capacity (B(max)) was 275 fmol/mg protein. Mg2+ and ruthenium red inhibited [3H]ryanodine binding (IC50 = 1.4 nM and 0.5 μM, respectively). 4-Chloro-3-ethylphenol enhanced the binding of [3H]ryanodine 2.5-fold; whereas ATP and caffeine were much less efficacious toward activating Ry3R (56% and 18% maximal enhancement, respectively). Bastadin, a navel modulator of the 12-kDa FK506 binding protein· RyR complex, increased [3H]ryanodine binding 3-4-fold by enhancing K(d). The immunosuppressant FK506 enhanced [3H]ryanodine receptor occupancy at >100 μM and antagonized the action of bastadin, suggesting that an immunophilin modulates Ry3R in parotid acini. These results suggest that Ry3R may play an important role in Ca2+ homeostasis in mouse parotid acini.

Original languageEnglish (US)
Pages (from-to)15687-15696
Number of pages10
JournalJournal of Biological Chemistry
Volume272
Issue number25
DOIs
StatePublished - Jun 20 1997

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Ryanodine
Ryanodine Receptor Calcium Release Channel
Binding Sites
Modulation
Immunophilins
Tacrolimus Binding Proteins
Membranes
Ruthenium Red
Tacrolimus
Immunosuppressive Agents
Caffeine
Modulators
Inhibitory Concentration 50
Protein Isoforms
Homeostasis
Salts
Adenosine Triphosphate
Temperature
Proteins

ASJC Scopus subject areas

  • Biochemistry

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Ryanodine receptor type III (Ry3R) identification in mouse parotid acini. Properties and modulation of [3H]ryanodine-binding sites. / DiJulio, Dennis H.; Watson, Eileen L.; Pessah, Isaac N; Jacobson, Kerry L.; Ott, Sabrina M.; Buck, Edmond D.; Singh, Jean C.

In: Journal of Biological Chemistry, Vol. 272, No. 25, 20.06.1997, p. 15687-15696.

Research output: Contribution to journalArticle

DiJulio, Dennis H. ; Watson, Eileen L. ; Pessah, Isaac N ; Jacobson, Kerry L. ; Ott, Sabrina M. ; Buck, Edmond D. ; Singh, Jean C. / Ryanodine receptor type III (Ry3R) identification in mouse parotid acini. Properties and modulation of [3H]ryanodine-binding sites. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 25. pp. 15687-15696.
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abstract = "Immunoblot analysis and [3H]ryanodine binding were used to characterize and identify ryanodine receptors (RyRs) in nonexcitable mouse parotid acini. Western analysis revealed ryanodine receptor type III (Ry3R) to be the only detectable isoform in parotid microsomal membranes. Binding of [3H]ryanodine to microsomal fractions was dependent on Ca2+, salt, pH, and temperature. At 23 °C, and in the presence of 0.5 M KCl and 100 μM Ca2+, [3H]ryanodine bound specifically to membranes with high affinity (K(d) = 6 nM); maximum binding capacity (B(max)) was 275 fmol/mg protein. Mg2+ and ruthenium red inhibited [3H]ryanodine binding (IC50 = 1.4 nM and 0.5 μM, respectively). 4-Chloro-3-ethylphenol enhanced the binding of [3H]ryanodine 2.5-fold; whereas ATP and caffeine were much less efficacious toward activating Ry3R (56{\%} and 18{\%} maximal enhancement, respectively). Bastadin, a navel modulator of the 12-kDa FK506 binding protein· RyR complex, increased [3H]ryanodine binding 3-4-fold by enhancing K(d). The immunosuppressant FK506 enhanced [3H]ryanodine receptor occupancy at >100 μM and antagonized the action of bastadin, suggesting that an immunophilin modulates Ry3R in parotid acini. These results suggest that Ry3R may play an important role in Ca2+ homeostasis in mouse parotid acini.",
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