Ryanodine induces maturation of embryonic acetylcholinesterase forms in cultured quail myotubes

Isaac N Pessah, Pamela S. Nieberg, Barry W. Wilson

Research output: Contribution to journalArticle

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Abstract

[3H]Ryanodine is shown to specifically bind to cultured myotubes from 10 day quail embryo pectorals. The binding of [3H]ryanodine increases in a time-dependent manner reaching 38 ±3 fmol/mg protein at 4 h. A level of theophylline (THEO; 5mM) that induces propagated wave-like contractures, doubles the capacity of the myotubes to bind [3H]ryanodine (78 ± 7 fmol/mg protein at 4 h). Polycationic ruthenium red (100μM) only partially inhibits (56%) [3H]ryanodine-binding, whereas the membrane permeable channel antagonist [2,6-dichloro-4-dimethyl-amino-phenyl]-isopropylamine (20μM) inhibits occupancy >80%. Ryanodine (10μM) interferes with THEO-induced contractures. Pretreatment with micromolar ryanodine for 48 h, followed by washout for 48 h, causes a persistent decrease in [3H]ryanodine-binding sites. Persistent [3H]ryanodine receptor blockade coincides with a dramatic shift in AChE forms found in the myotubes. A transition from the embryonic 4S and 7S globular forms to the 20S collagen-tailed (adult) form is evident within 12 hr exposure to ryanodine and progresses after removal of the alkaloid from the culture medium, mimicking the transition that normally occurs during myocyte maturation in vivo. These results suggest that SR Ca++ movements and excitation-contraction coupling may, at least in part, contribute to AChE maturation.

Original languageEnglish (US)
Pages (from-to)1279-1285
Number of pages7
JournalLife Sciences
Volume52
Issue number15
StatePublished - 1993

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Ryanodine
Quail
Skeletal Muscle Fibers
Acetylcholinesterase
Contracture
Ruthenium Red
Excitation Contraction Coupling
Ryanodine Receptor Calcium Release Channel
Theophylline
Ion Channels
Alkaloids
Muscle Cells
Culture Media
Proteins
Collagen
Embryonic Structures
Binding Sites
Membranes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Ryanodine induces maturation of embryonic acetylcholinesterase forms in cultured quail myotubes. / Pessah, Isaac N; Nieberg, Pamela S.; Wilson, Barry W.

In: Life Sciences, Vol. 52, No. 15, 1993, p. 1279-1285.

Research output: Contribution to journalArticle

Pessah, Isaac N ; Nieberg, Pamela S. ; Wilson, Barry W. / Ryanodine induces maturation of embryonic acetylcholinesterase forms in cultured quail myotubes. In: Life Sciences. 1993 ; Vol. 52, No. 15. pp. 1279-1285.
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