RXRA and HSPA5 map to the telomeric end of dog chromosome 9

P. Werner, M. G. Raducha, U. Prociuk, Leslie A Lyons, J. S. Kehler, P. S. Henthorn, D. F. Patterson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Previous results showed that loci from human chromosome 17q (HSA17q) map to the centromeric two-thirds of dog chromosome 9 (CFA9). In these studies fluorescence in situ hybridization (FISH) using a human total chromosome 17 painting probe, indicated that the telomeric one-third of CFA9 must have homology to one or more human chromosomes other than HSA17. Here we report that this distal part of CFA9 contains a segment syntenic to the telomeric end of HSA9q and mouse chromosome 2 (MMU2). The gene loci encoding retinoid X receptor, alpha (RXRA) and heat shock protein 5 (HSPA5 or GRP78), which are found on HSA9q34 and MMU2, occupy a region on CFA9 distal to NF1 and CRYBA1. FISH of a canine specific genomic cosmid clone for RXRA demonstrated the more telomeric localization of this locus to NF1 on CFA9. A linkage map developed for the distal region of CFA9 included: NF1-(2.7 CM)-CRYBA1-(6.5 CM)-RXRA-(22 CM)-HSPA5. The next best order, RXRA-NF1-CRYBA1-HSPA5 with a difference in the log odds of 1.43 does not correspond to our findings with FISH. The most probable map order places HSPA5 distal to RXRA on CFA9 whereas in humans it lies centromeric of RXRA on HSA9q34.

Original languageEnglish (US)
Pages (from-to)220-223
Number of pages4
JournalAnimal Genetics
Volume29
Issue number3
DOIs
StatePublished - Jun 1998
Externally publishedYes

Fingerprint

Retinoid X Receptor alpha
Chromosomes, Human, Pair 9
retinoids
Dogs
chromosomes
receptors
dogs
Human Chromosomes
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
loci
Chromosome Painting
Cosmids
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 2
heat shock proteins
chromosome mapping
Canidae
Clone Cells
clones

Keywords

  • Canine
  • CFA9
  • Comparative gene mapping
  • Dog chromosome
  • HSPA5
  • RXRA

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

Werner, P., Raducha, M. G., Prociuk, U., Lyons, L. A., Kehler, J. S., Henthorn, P. S., & Patterson, D. F. (1998). RXRA and HSPA5 map to the telomeric end of dog chromosome 9. Animal Genetics, 29(3), 220-223. https://doi.org/10.1046/j.1365-2052.1998.00328.x

RXRA and HSPA5 map to the telomeric end of dog chromosome 9. / Werner, P.; Raducha, M. G.; Prociuk, U.; Lyons, Leslie A; Kehler, J. S.; Henthorn, P. S.; Patterson, D. F.

In: Animal Genetics, Vol. 29, No. 3, 06.1998, p. 220-223.

Research output: Contribution to journalArticle

Werner, P, Raducha, MG, Prociuk, U, Lyons, LA, Kehler, JS, Henthorn, PS & Patterson, DF 1998, 'RXRA and HSPA5 map to the telomeric end of dog chromosome 9', Animal Genetics, vol. 29, no. 3, pp. 220-223. https://doi.org/10.1046/j.1365-2052.1998.00328.x
Werner P, Raducha MG, Prociuk U, Lyons LA, Kehler JS, Henthorn PS et al. RXRA and HSPA5 map to the telomeric end of dog chromosome 9. Animal Genetics. 1998 Jun;29(3):220-223. https://doi.org/10.1046/j.1365-2052.1998.00328.x
Werner, P. ; Raducha, M. G. ; Prociuk, U. ; Lyons, Leslie A ; Kehler, J. S. ; Henthorn, P. S. ; Patterson, D. F. / RXRA and HSPA5 map to the telomeric end of dog chromosome 9. In: Animal Genetics. 1998 ; Vol. 29, No. 3. pp. 220-223.
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abstract = "Previous results showed that loci from human chromosome 17q (HSA17q) map to the centromeric two-thirds of dog chromosome 9 (CFA9). In these studies fluorescence in situ hybridization (FISH) using a human total chromosome 17 painting probe, indicated that the telomeric one-third of CFA9 must have homology to one or more human chromosomes other than HSA17. Here we report that this distal part of CFA9 contains a segment syntenic to the telomeric end of HSA9q and mouse chromosome 2 (MMU2). The gene loci encoding retinoid X receptor, alpha (RXRA) and heat shock protein 5 (HSPA5 or GRP78), which are found on HSA9q34 and MMU2, occupy a region on CFA9 distal to NF1 and CRYBA1. FISH of a canine specific genomic cosmid clone for RXRA demonstrated the more telomeric localization of this locus to NF1 on CFA9. A linkage map developed for the distal region of CFA9 included: NF1-(2.7 CM)-CRYBA1-(6.5 CM)-RXRA-(22 CM)-HSPA5. The next best order, RXRA-NF1-CRYBA1-HSPA5 with a difference in the log odds of 1.43 does not correspond to our findings with FISH. The most probable map order places HSPA5 distal to RXRA on CFA9 whereas in humans it lies centromeric of RXRA on HSA9q34.",
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