RXR ligands modulate thyroid hormone signaling competence in young xenopus laevis tadpoles

Brenda J. Mengeling, Michael L. Goodson, John Furlow

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Appropriate thyroid hormone (TH) signaling through thyroid hormone receptors (TRs) is essential for vertebrate development. Amphibian metamorphosis is initiated and sustained through the action of TH on TRs, which are conserved across vertebrates. TRs heterodimerize with retinoid X receptors (RXRs) on thyroid hormone response elements (TREs) in the genome; however, in most cell line and adult animal studies, RXR ligands do not affect expression of TR target genes. We used a quantitative, precocious metamorphosis assay to interrogate the effects of the RXR agonist bexarotene (Bex) and the RXR antagonist UVI 3003 (UVI) on T3-induced resorption phenotypes in Xenopus laevis tadpoles 1 week postfertilization. Bex potentiated gill and tail resorption, and UVI abrogated T3 action. These results held in transgenic tadpoles bearing a TRE-driven luciferase reporter. Therefore, we used poly-A-primed RNA sequencing transcriptomic analysis to determine their effects on T3-induced gene expression. We also assayed the environmental pollutant tributyltin (TBT), which is an RXR agonist. We found that the proteases that carry out resorption were potentiated by Bex and TBT but were not significantly inhibited by UVI. However, several transcription factors from multiple families (sox4, fosl2, mxd1, mafb, nfib) were all inhibited by UVI and potentiated by Bex and TBT. All required T3 for induction. Time course analysis of gene expression showed that although the agonists could potentiate within 12 hours, the antagonist response lagged. These data indicate that the agonists and antagonist are not necessarily functioning through the same mechanism and suggest that RXR liganding may modulate TH competence in metamorphic signaling.

Original languageEnglish (US)
Pages (from-to)2576-2595
Number of pages20
JournalEndocrinology
Volume159
Issue number7
DOIs
StatePublished - Jul 1 2018

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Retinoid X Receptors
Xenopus laevis
Thyroid Hormones
Mental Competency
Larva
Thyroid Hormone Receptors
Ligands
Response Elements
Vertebrates
RNA Sequence Analysis
Gene Expression
Environmental Pollutants
Amphibians
Luciferases
Tail
Peptide Hydrolases
Transcription Factors
Genome
Phenotype
Cell Line

ASJC Scopus subject areas

  • Endocrinology

Cite this

RXR ligands modulate thyroid hormone signaling competence in young xenopus laevis tadpoles. / Mengeling, Brenda J.; Goodson, Michael L.; Furlow, John.

In: Endocrinology, Vol. 159, No. 7, 01.07.2018, p. 2576-2595.

Research output: Contribution to journalArticle

Mengeling, Brenda J. ; Goodson, Michael L. ; Furlow, John. / RXR ligands modulate thyroid hormone signaling competence in young xenopus laevis tadpoles. In: Endocrinology. 2018 ; Vol. 159, No. 7. pp. 2576-2595.
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