Abstract
Retinoids are well-characterized differentiation and anti-proliferation agents. The functional role of retinoid x receptor alpha (RXRα) in cell proliferation and cell cycle regulation is not well understood. Using human Hep3B cell line, we showed that the mRNA level of RXRα was closely associated with cell growth. RXRα mRNA expression elevated along with the proliferation of Hep3B cells. This association was most evident in RXRα and was also noted with retinoic acid (RA) receptor α (RARα), but not found in other RARs and RXRs. The expression of RXRα and cyclin A mRNA was co-regulated when Hep3B cells were cultured in serum-free medium. The mRNA levels of RXRα and cyclin A appeared to be highest in G1/S phase in Hep3B cells treated by aphidicolin. Taken together, our data suggest that RXRα may be actively involved in cell proliferation and cell cycle regulation in Hep3B cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 24-28 |
| Number of pages | 5 |
| Journal | Experimental and Molecular Pathology |
| Volume | 76 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2004 |
| Externally published | Yes |
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Keywords
- Cell cycle
- Cyclin A
- Hepatoma
- Proliferation
- Retinoic acid
- Retinoid x receptor
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Biology
- Pathology and Forensic Medicine
Cite this
RXRα mRNA expression is associated with cell proliferation and cell cycle regulation in Hep3B cell. / Wu, Yong; Cai, Yan; Aguilo, Jonathan; Dai, Tiane; Ao, Yan; Wan, Yu-Jui Yvonne.
In: Experimental and Molecular Pathology, Vol. 76, No. 1, 02.2004, p. 24-28.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RXRα mRNA expression is associated with cell proliferation and cell cycle regulation in Hep3B cell
AU - Wu, Yong
AU - Cai, Yan
AU - Aguilo, Jonathan
AU - Dai, Tiane
AU - Ao, Yan
AU - Wan, Yu-Jui Yvonne
PY - 2004/2
Y1 - 2004/2
N2 - Retinoids are well-characterized differentiation and anti-proliferation agents. The functional role of retinoid x receptor alpha (RXRα) in cell proliferation and cell cycle regulation is not well understood. Using human Hep3B cell line, we showed that the mRNA level of RXRα was closely associated with cell growth. RXRα mRNA expression elevated along with the proliferation of Hep3B cells. This association was most evident in RXRα and was also noted with retinoic acid (RA) receptor α (RARα), but not found in other RARs and RXRs. The expression of RXRα and cyclin A mRNA was co-regulated when Hep3B cells were cultured in serum-free medium. The mRNA levels of RXRα and cyclin A appeared to be highest in G1/S phase in Hep3B cells treated by aphidicolin. Taken together, our data suggest that RXRα may be actively involved in cell proliferation and cell cycle regulation in Hep3B cells.
AB - Retinoids are well-characterized differentiation and anti-proliferation agents. The functional role of retinoid x receptor alpha (RXRα) in cell proliferation and cell cycle regulation is not well understood. Using human Hep3B cell line, we showed that the mRNA level of RXRα was closely associated with cell growth. RXRα mRNA expression elevated along with the proliferation of Hep3B cells. This association was most evident in RXRα and was also noted with retinoic acid (RA) receptor α (RARα), but not found in other RARs and RXRs. The expression of RXRα and cyclin A mRNA was co-regulated when Hep3B cells were cultured in serum-free medium. The mRNA levels of RXRα and cyclin A appeared to be highest in G1/S phase in Hep3B cells treated by aphidicolin. Taken together, our data suggest that RXRα may be actively involved in cell proliferation and cell cycle regulation in Hep3B cells.
KW - Cell cycle
KW - Cyclin A
KW - Hepatoma
KW - Proliferation
KW - Retinoic acid
KW - Retinoid x receptor
UR - http://www.scopus.com/inward/record.url?scp=0346040263&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0346040263&partnerID=8YFLogxK
U2 - 10.1016/j.yexmp.2003.10.009
DO - 10.1016/j.yexmp.2003.10.009
M3 - Article
C2 - 14738865
AN - SCOPUS:0346040263
VL - 76
SP - 24
EP - 28
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
SN - 0014-4800
IS - 1
ER -