Rotationally oscillating drill (Ros-Drill©) for mouse ICSI without using mercury

Ali Fuat Ergenc; Ming Wen Li; Mehmet Toner; John D. Biggers; Kevin C K Lloyd; Nejat Olgac

doi:10.1002/mrd.20919

Rotationally oscillating drill (Ros-Drill©) for mouse ICSI without using mercury

Ali Fuat Ergenc, Ming Wen Li, Mehmet Toner, John D. Biggers, Kevin C K Lloyd, Nejat Olgac

Surgery

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An important and remaining problem with this technique, however, is that it requires small amounts of mercury to stabilize the pipette tip when piezoelectric force pulses are applied. To eliminate this problem we developed and tested a completely different and mercury-free technology, called the "Ros-Drill

Original language	English (US)
Pages (from-to)	1744-1751
Number of pages	8
Journal	Molecular Reproduction and Development
Volume	75
Issue number	12
DOIs	https://doi.org/10.1002/mrd.20919
State	Published - Dec 2008

Keywords

Assisted reproduction
ICSI
Microinjection
Micromanipulation

ASJC Scopus subject areas

Genetics
Developmental Biology
Cell Biology

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AU - Toner, Mehmet

AU - Biggers, John D.

AU - Lloyd, Kevin C K

AU - Olgac, Nejat

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AB - Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An important and remaining problem with this technique, however, is that it requires small amounts of mercury to stabilize the pipette tip when piezoelectric force pulses are applied. To eliminate this problem we developed and tested a completely different and mercury-free technology, called the "Ros-Drill

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KW - Microinjection

KW - Micromanipulation

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Rotationally oscillating drill (Ros-Drill©) for mouse ICSI without using mercury

Abstract

Keywords

ASJC Scopus subject areas

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