Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: Bimodal system for intravascular applications

Dinglong Ma, Julien Bec, Diego R. Yankelevich, Dimitris Gorpas, Hussain Fatakdawala, Laura Marcu

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We report the development and validation of a hybrid intravascular diagnostic system combining multispectral fluorescence lifetime imaging (FLIm) and intravascular ultrasound (IVUS) for cardiovascular imaging applications. A prototype FLIm system based on fluorescence pulse sampling technique providing information on artery biochemical composition was integrated with a commercial IVUS system providing information on artery morphology. A customized 3-Fr bimodal catheter combining a rotational side-view fiberoptic and a 40-MHz IVUS transducer was constructed for sequential helical scanning (rotation and pullback) of tubular structures. Validation of this bimodal approach was conducted in pig heart coronary arteries. Spatial resolution, fluorescence detection efficiency, pulse broadening effect, and lifetime measurement variability of the FLIm system were systematically evaluated. Current results show that this system is capable of temporarily resolving the fluorescence emission simultaneously in multiple spectral channels in a single pullback sequence. Accurate measurements of fluorescence decay characteristics from arterial segments can be obtained rapidly (e.g., 20mmin 5 s), and accurate co-registration of fluorescence and ultrasound features can be achieved. The current finding demonstrates the compatibility of FLIm instrumentation with in vivo clinical investigations and its potential to complement conventional IVUS during catheterization procedures.

Original languageEnglish (US)
Article number140097R
JournalJournal of Biomedical Optics
Volume19
Issue number6
DOIs
StatePublished - 2014

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Keywords

  • bimodal catheter
  • fluorescence lifetime spectroscopy and imaging
  • intravascular imaging
  • intravascular ultrasound

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Medicine(all)

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