Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: Bimodal system for intravascular applications

Dinglong Ma, Julien Bec, Diego R. Yankelevich, Dimitris Gorpas, Hussain Fatakdawala, Laura Marcu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We report the development and validation of a hybrid intravascular diagnostic system combining multispectral fluorescence lifetime imaging (FLIm) and intravascular ultrasound (IVUS) for cardiovascular imaging applications. A prototype FLIm system based on fluorescence pulse sampling technique providing information on artery biochemical composition was integrated with a commercial IVUS system providing information on artery morphology. A customized 3-Fr bimodal catheter combining a rotational side-view fiberoptic and a 40-MHz IVUS transducer was constructed for sequential helical scanning (rotation and pullback) of tubular structures. Validation of this bimodal approach was conducted in pig heart coronary arteries. Spatial resolution, fluorescence detection efficiency, pulse broadening effect, and lifetime measurement variability of the FLIm system were systematically evaluated. Current results show that this system is capable of temporarily resolving the fluorescence emission simultaneously in multiple spectral channels in a single pullback sequence. Accurate measurements of fluorescence decay characteristics from arterial segments can be obtained rapidly (e.g., 20mmin 5 s), and accurate co-registration of fluorescence and ultrasound features can be achieved. The current finding demonstrates the compatibility of FLIm instrumentation with in vivo clinical investigations and its potential to complement conventional IVUS during catheterization procedures.

Original languageEnglish (US)
Article number140097R
JournalJournal of Biomedical Optics
Volume19
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Optical Imaging
Fluorescence
Ultrasonics
Imaging techniques
life (durability)
fluorescence
Pulse
arteries
Arteries
Imaging systems
intravascular system
Transducers
Information Systems
Catheterization
Ultrasonography
Coronary Vessels
catheterization
Swine
Catheters
swine

Keywords

  • bimodal catheter
  • fluorescence lifetime spectroscopy and imaging
  • intravascular imaging
  • intravascular ultrasound

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Medicine(all)

Cite this

Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound : Bimodal system for intravascular applications. / Ma, Dinglong; Bec, Julien; Yankelevich, Diego R.; Gorpas, Dimitris; Fatakdawala, Hussain; Marcu, Laura.

In: Journal of Biomedical Optics, Vol. 19, No. 6, 140097R, 2014.

Research output: Contribution to journalArticle

Ma, Dinglong ; Bec, Julien ; Yankelevich, Diego R. ; Gorpas, Dimitris ; Fatakdawala, Hussain ; Marcu, Laura. / Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound : Bimodal system for intravascular applications. In: Journal of Biomedical Optics. 2014 ; Vol. 19, No. 6.
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