TY - JOUR
T1 - Rotatable Aggregation-Induced-Emission/Aggregation-Caused-Quenching Ratio Strategy for Real-Time Tracking Nanoparticle Dynamics
AU - Wu, Hao
AU - Zhang, Lu
AU - Yang, Jinfan
AU - Bo, Ruonan
AU - Du, Hongxu
AU - Lin, Kai
AU - Zhang, Dalin
AU - Ramachandran, Mythili
AU - Shen, Yingbin
AU - Xu, Yangxi
AU - Xue, Xiangdong
AU - Ma, Zhao
AU - Lindstrom, Aaron Raymond
AU - Carney, Randy
AU - Lin, Tzu Yin
AU - Li, Yuanpei
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Real-time tracking of the dynamics change of self-assembled nanostructures in physiological environments is crucial to improving their delivery efficiency and therapeutic effects. However, such tracking is impeded by the complex biological microenvironment leading to inhomogeneous distribution. A rotatable fluorescent ratio strategy is introduced that integrates aggregation-induced emission (AIE) and aggregation-caused quenching (ACQ) into one nanostructured system, termed AIE and ACQ fluorescence ratio (AAR). Following this strategy, an advanced probe, PEG5k-TPE4-ICGD4 (PTI), is developed to track the dynamics change. The extremely sharp fluorescent changes (up to 4008-fold) in AAR allowed for the clear distinguishing and localization of the intact state and diverse dissociated states. The spatiotemporal distribution and structural dynamics of the PTI micelles can be tracked, quantitatively analyzed in living cells and animal tissue by the real-time ratio map, and be used to monitor other responsive nanoplatforms. With this method, the dynamics of nanoparticle in different organelles are able to be investigated and validated by transmission electron microscopy. This novel strategy is generally applicable to many self-assembled nanostructures for understanding delivery mechanism in living systems, ultimately to enhance their performance in biomedical applications.
AB - Real-time tracking of the dynamics change of self-assembled nanostructures in physiological environments is crucial to improving their delivery efficiency and therapeutic effects. However, such tracking is impeded by the complex biological microenvironment leading to inhomogeneous distribution. A rotatable fluorescent ratio strategy is introduced that integrates aggregation-induced emission (AIE) and aggregation-caused quenching (ACQ) into one nanostructured system, termed AIE and ACQ fluorescence ratio (AAR). Following this strategy, an advanced probe, PEG5k-TPE4-ICGD4 (PTI), is developed to track the dynamics change. The extremely sharp fluorescent changes (up to 4008-fold) in AAR allowed for the clear distinguishing and localization of the intact state and diverse dissociated states. The spatiotemporal distribution and structural dynamics of the PTI micelles can be tracked, quantitatively analyzed in living cells and animal tissue by the real-time ratio map, and be used to monitor other responsive nanoplatforms. With this method, the dynamics of nanoparticle in different organelles are able to be investigated and validated by transmission electron microscopy. This novel strategy is generally applicable to many self-assembled nanostructures for understanding delivery mechanism in living systems, ultimately to enhance their performance in biomedical applications.
KW - aggregation-caused quenching
KW - aggregation-induced emission
KW - fluorescence ratios
KW - micelles
KW - nanostructures
UR - http://www.scopus.com/inward/record.url?scp=85079709391&partnerID=8YFLogxK
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U2 - 10.1002/adfm.201910348
DO - 10.1002/adfm.201910348
M3 - Article
AN - SCOPUS:85079709391
JO - Advanced Materials for Optics and Electronics
JF - Advanced Materials for Optics and Electronics
SN - 1057-9257
M1 - 1910348
ER -