RosE represses Std fimbrial expression in Salmonella enterica serotype Typhimurium

Daniela Chessa, Maria G. Winter, Sean Paul Nuccio, Çagla Tükel, Andreas J Baumler

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The Salmonella enterica serotype Typhimurium (S. typhimurium) genome contains a large repertoire of putative fimbrial operons that remain poorly characterized because they are not expressed in vitro. In this study, insertions that induced expression of the putative stdABCD fimbrial operon were identified from a random bank of transposon mutants by screening with immuno-magnetic particles for ligand expression (SIMPLE). Transposon insertions upstream of csgC and lrhA or within dam, setB and STM4463 (renamed rosE) resulted in expression of StdA and its assembly into fimbrial filaments on the cell surface. RosE is a novel negative regulator of Std fimbrial expression as indicated by its repression of a std::lacZ reporter construct and by binding of the purified protein to a DNA region upstream of the stdA start codon. Expression of Std fimbriae in the rosE mutant resulted in increased attachment of S. typhimurium to human colonic epithelial cell lines (T-84 and CaCo-2). A rosE mutant exhibited a reduced ability to compete with virulent S. typhimurium for colonization of murine organs, while no defect was observed when both competing strains carried a stdAB deletion. These data suggest that a tight control of Std fimbrial expression mediated by RosE is required during host pathogen interaction.

Original languageEnglish (US)
Pages (from-to)573-587
Number of pages15
JournalMolecular Microbiology
Volume68
Issue number3
DOIs
StatePublished - May 2008

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Salmonella enterica
Operon
Host-Pathogen Interactions
Initiator Codon
Carrier Proteins
Epithelial Cells
Genome
Ligands
Cell Line
DNA
Serogroup

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

RosE represses Std fimbrial expression in Salmonella enterica serotype Typhimurium. / Chessa, Daniela; Winter, Maria G.; Nuccio, Sean Paul; Tükel, Çagla; Baumler, Andreas J.

In: Molecular Microbiology, Vol. 68, No. 3, 05.2008, p. 573-587.

Research output: Contribution to journalArticle

Chessa, Daniela ; Winter, Maria G. ; Nuccio, Sean Paul ; Tükel, Çagla ; Baumler, Andreas J. / RosE represses Std fimbrial expression in Salmonella enterica serotype Typhimurium. In: Molecular Microbiology. 2008 ; Vol. 68, No. 3. pp. 573-587.
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