Abstract
Vaccination with recombinant outer surface protein A (OspA) has been shown to protect mice from infection with Borrelia burgdorferi, the Lyme disease agent. To determine whether antibodies to B. burgdorferi proteins other than OspA are involved in protective immunity, antibodies to OspA were removed from protective anti-B. burgdorferi serum; the residual serum was still protective. Absorption of OspA and OspB antibodies from anti-B. burgdorferi serum eliminated the protective effect. Therefore, active immunization experiments were performed to determine the roles of OspB and flagellin in protective immunity and to determine whether protective immunity induced by OspA is dose dependent. Active immunization with recombinant OspA protected mice from infection with an inoculum of 104 spirochetes, but this protection could be overcome with a challenge of 107 spirochetes; OspB protected mice from infection with an inoculum of 103 spirochetes but was insufficient to fully protect against 104 organisms; and immunization with flagellin had no protective effect. These studies suggest that OspA and OspB, but not flagellin, play roles in protective immunity to spirochete infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 657-661 |
| Number of pages | 5 |
| Journal | Infection and Immunity |
| Volume | 60 |
| Issue number | 2 |
| State | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Roles of OspA, OspB, and flagellin in protective immunity to Lyme borreliosis in laboratory mice. / Fikrig, E.; Barthold, Stephen W; Marcantonio, N.; Deponte, K.; Kantor, F. S.; Flavell, R. A.
In: Infection and Immunity, Vol. 60, No. 2, 1991, p. 657-661.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Roles of OspA, OspB, and flagellin in protective immunity to Lyme borreliosis in laboratory mice
AU - Fikrig, E.
AU - Barthold, Stephen W
AU - Marcantonio, N.
AU - Deponte, K.
AU - Kantor, F. S.
AU - Flavell, R. A.
PY - 1991
Y1 - 1991
N2 - Vaccination with recombinant outer surface protein A (OspA) has been shown to protect mice from infection with Borrelia burgdorferi, the Lyme disease agent. To determine whether antibodies to B. burgdorferi proteins other than OspA are involved in protective immunity, antibodies to OspA were removed from protective anti-B. burgdorferi serum; the residual serum was still protective. Absorption of OspA and OspB antibodies from anti-B. burgdorferi serum eliminated the protective effect. Therefore, active immunization experiments were performed to determine the roles of OspB and flagellin in protective immunity and to determine whether protective immunity induced by OspA is dose dependent. Active immunization with recombinant OspA protected mice from infection with an inoculum of 104 spirochetes, but this protection could be overcome with a challenge of 107 spirochetes; OspB protected mice from infection with an inoculum of 103 spirochetes but was insufficient to fully protect against 104 organisms; and immunization with flagellin had no protective effect. These studies suggest that OspA and OspB, but not flagellin, play roles in protective immunity to spirochete infection.
AB - Vaccination with recombinant outer surface protein A (OspA) has been shown to protect mice from infection with Borrelia burgdorferi, the Lyme disease agent. To determine whether antibodies to B. burgdorferi proteins other than OspA are involved in protective immunity, antibodies to OspA were removed from protective anti-B. burgdorferi serum; the residual serum was still protective. Absorption of OspA and OspB antibodies from anti-B. burgdorferi serum eliminated the protective effect. Therefore, active immunization experiments were performed to determine the roles of OspB and flagellin in protective immunity and to determine whether protective immunity induced by OspA is dose dependent. Active immunization with recombinant OspA protected mice from infection with an inoculum of 104 spirochetes, but this protection could be overcome with a challenge of 107 spirochetes; OspB protected mice from infection with an inoculum of 103 spirochetes but was insufficient to fully protect against 104 organisms; and immunization with flagellin had no protective effect. These studies suggest that OspA and OspB, but not flagellin, play roles in protective immunity to spirochete infection.
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M3 - Article
VL - 60
SP - 657
EP - 661
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 2
ER -