Roles of neutrophil β2 integrins in kinetics of bacteremia, extravasation, and tick acquisition of Anaplasma phagocytophila in mice

Dori L. Borjesson, Scott I. Simon, Emir Hodzic, Hilde E V DeCock, Christie M. Ballantyne, Stephen W. Barthold

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22 Citations (Scopus)

Abstract

Tick saliva contains anti-inflammatory and immunosuppressive substances that facilitate blood feeding and enhance tick-vectored pathogen transmission, including Anaplasma phagocytophila, an etiologic agent of granulocytic ehrlichiosis. As such, inflammation at a tick-feeding site is strikingly different than that typically observed at other sites of inflammation. Up-regulation of CD11b/CD18 occurs in host granulocytes following interaction or infection with A phagocytophila, and the absence of CD11b/CD18 results in early increases in bacteremia. We hypothesized that β2 integrin-dependent infection kinetics and leukocyte extravasation are important determinants of neutrophil trafficking to, and pathogen acquisition at, tick-feeding sites. A phagocytophila infection kinetics were evaluated in CD11a/CD18, CD11b/CD18, and CD18 knock-out mice using quantitative polymerase chain reaction (PCR) of blood, ticks, and skin biopsies in conjunction with histopathology. A marked increase in the rate of A phagocytophila infection of neutrophils and pathogen burden in blood followed tick feeding. Infection kinetics were modified by β2 integrin expression and systemic neutrophil counts. Significant neutrophil-pathogen trafficking was observed to both suture and tick sites. Despite the prominent role for β2 integrins in neutrophil arrest in flowing blood, successful pathogen acquisition by ticks occurred in the absence of β2 integrins. Establishment of feeding pools that rely less on leukocyte trafficking and more on small hemorrhages may explain the ready amplification of A phagocytophila DNA from ticks infested on CD11/CD18-deficient mouse strains.

Original languageEnglish (US)
Pages (from-to)3257-3264
Number of pages8
JournalBlood
Volume101
Issue number8
DOIs
StatePublished - Apr 15 2003

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Anaplasma phagocytophilum
Ticks
Pathogens
Bacteremia
Integrins
Neutrophils
Blood
Kinetics
Infection
Biopsy
Polymerase chain reaction
Immunosuppressive Agents
Leukocytes
Amplification
Skin
Anti-Inflammatory Agents
Ehrlichiosis
Inflammation
Infectious Disease Transmission
DNA

ASJC Scopus subject areas

  • Hematology

Cite this

Roles of neutrophil β2 integrins in kinetics of bacteremia, extravasation, and tick acquisition of Anaplasma phagocytophila in mice. / Borjesson, Dori L.; Simon, Scott I.; Hodzic, Emir; DeCock, Hilde E V; Ballantyne, Christie M.; Barthold, Stephen W.

In: Blood, Vol. 101, No. 8, 15.04.2003, p. 3257-3264.

Research output: Contribution to journalArticle

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