Role of the CaMKII/NMDA receptor complex in the maintenance of synaptic strength

Magdalena Sanhueza, German Fernandez-Villalobos, Ivar S. Stein, Gyulnara Kasumova, Peng Zhang, K. Ulrich Bayer, Nikolai Otmakhov, Johannes W Hell, John Lisman

Research output: Contribution to journalArticlepeer-review

185 Scopus citations


During long-term potentiation (LTP), synapses undergo stable changes in synaptic strength. The molecular memory processes that maintain strength have not been identified. One hypothesis is that the complex formed by the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and the NMDA-type glutamate receptor (NMDAR) is a molecular memory at the synapse. To establish a molecule as a molecular memory, it must be shown that interfering with the molecule produces a persistent reversal of LTP. We used the CN class of peptides that inhibit Ca MKII binding to the NR2B subunit in vitro to test this prediction in rat hippocampal slices. We found that CN peptides can reverse saturated LTP, allowing additional LTP to be induced. The peptide also produced a persistent reduction in basal transmission. We then tested whether CN compounds actually affect Ca MKII binding in living cells. Application of CN peptide to slice cultures reduced the amount of Ca MKII concentrated in spines, consistent with delocalization of the kinase from a binding partner in the spine. To more specifically assay the binding of CaMKII to the NMDAR, we used coimmunoprecipitation methods. We found that CN peptide decreased synaptic strength only at concentrations necessary to disrupt the Ca MKII/NMDAR complex, but not at lower concentrations sufficient to inhibit Ca MKII activity. Importantly, both the reduction of the complex and the reduction of synaptic strength persisted after removal of the inhibitor. These results support the hypothesis that the Ca MKII/NMDAR complex has switch-like properties that are important in the maintenance of synaptic strength.

Original languageEnglish (US)
Pages (from-to)9170-9178
Number of pages9
JournalJournal of Neuroscience
Issue number25
StatePublished - Jun 22 2011

ASJC Scopus subject areas

  • Neuroscience(all)


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