TY - JOUR
T1 - Role of stimulatory guanine nucleotide binding protein (G(Sα)) in proliferation of PC-3M prostate cancer cells
AU - Chien, Jeremy
AU - Shah, Girish V.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Previous studies have shown that calcitonin-like immunoreactive substances are secreted by primary prostate cells. Furthermore, exogenously added calcitonin stimulates proliferation of androgen-responsive LnCaP cells. To examine the possible effect of calcitonin on growth of invasive prostate cancer cells, we tested its effects on proliferation of PC-3M cells. Calcitonin stimulated DNA synthesis of PC-3M cells in a dose-dependent fashion, and also stimulated adenylyl cyclase and protein kinase C activities. To further delineate the role of these signaling cascades in proliferation of PC-3M prostate cancer cells, we selectively activated these pathways by transfecting cDNAs expressing constitutively active forms of either G(s)α (G(s)α-QL) or G(q)α (G(q)α-QL). cDNAs expressing wild-type forms of G-proteins (G(s)α-WT and G(q)α-WT) were used as vehicle controls. G(q)α-QL transfectants exhibited growth inhibition and terminal differentiation. Those expressing G(s)α-QL exhibited a dramatic increase in growth rate. G(s)α-QL transfectants displayed an almost 3-fold increase in [3H]-thymidine incorporation and over a 4-fold increase in growth rate when compared with parental PC-3M cells or those expressing wild-type G(s)α (G(s)α-WT). The growth-promoting action of G(s)α-QL could not be mimicked by either 8-bromo cAMP or forskolin. However, nifedipine, a calcium channel antagonist, potently and selectively inhibited DNA synthesis in G(s)α-QL transfectants. These results suggest that the growth-promoting actions of G(s)α on PC-3M cells may be mediated by nifedipine-sensitive proliferative events. (C) 2001 Wiley-Liss, Inc.
AB - Previous studies have shown that calcitonin-like immunoreactive substances are secreted by primary prostate cells. Furthermore, exogenously added calcitonin stimulates proliferation of androgen-responsive LnCaP cells. To examine the possible effect of calcitonin on growth of invasive prostate cancer cells, we tested its effects on proliferation of PC-3M cells. Calcitonin stimulated DNA synthesis of PC-3M cells in a dose-dependent fashion, and also stimulated adenylyl cyclase and protein kinase C activities. To further delineate the role of these signaling cascades in proliferation of PC-3M prostate cancer cells, we selectively activated these pathways by transfecting cDNAs expressing constitutively active forms of either G(s)α (G(s)α-QL) or G(q)α (G(q)α-QL). cDNAs expressing wild-type forms of G-proteins (G(s)α-WT and G(q)α-WT) were used as vehicle controls. G(q)α-QL transfectants exhibited growth inhibition and terminal differentiation. Those expressing G(s)α-QL exhibited a dramatic increase in growth rate. G(s)α-QL transfectants displayed an almost 3-fold increase in [3H]-thymidine incorporation and over a 4-fold increase in growth rate when compared with parental PC-3M cells or those expressing wild-type G(s)α (G(s)α-WT). The growth-promoting action of G(s)α-QL could not be mimicked by either 8-bromo cAMP or forskolin. However, nifedipine, a calcium channel antagonist, potently and selectively inhibited DNA synthesis in G(s)α-QL transfectants. These results suggest that the growth-promoting actions of G(s)α on PC-3M cells may be mediated by nifedipine-sensitive proliferative events. (C) 2001 Wiley-Liss, Inc.
KW - G-protein-cell proliferation
KW - Prostate cancer
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U2 - 10.1002/1097-0215(20010101)91:1<46::AID-IJC1008>3.0.CO;2-0
DO - 10.1002/1097-0215(20010101)91:1<46::AID-IJC1008>3.0.CO;2-0
M3 - Article
C2 - 11149419
AN - SCOPUS:0035195434
VL - 91
SP - 46
EP - 54
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 1
ER -