Role of squalene in the organization of monolayers derived from lipid extracts of halobacterium salinarum

Sean F. Gilmore, Andrew I. Yao, Zipora Tietel, Tobias Kind, Marc T. Facciotti, Atul N. Parikh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have studied interfacial compressibility and lateral organization in monolayer configurations of total (squalene containing) and polar (squalene-devoid) lipid extracts of Halobacterium salinarum NRC-1, an extremely halophilic archaeon. Pressure-area isotherms derived from Langmuir experiments reveal that packing characteristics and elastic compressibility are strongly influenced by the presence of squalene in the total lipid extract. In conjunction with control experiments using mixtures of DPhPC and squalene, our results establish that the presence of squalene significantly extends elastic area compressibility of total lipid extracts, suggesting it has a role in facilitating tighter packing of archaeal lipid mixtures. Moreover, we find that squalene also influences spatial organization in archaeal membranes. Epifluorescence and atomic force microscopy characterization of Langmuir monolayers transferred onto solid hydrophilic substrates reveal an unusual domain morphology. Individual domains of microscopic dimensions (as well as their extended networks) exhibiting a peculiar bowl-like topography are evident in atomic force microscopy images. The tall rims outlining individual domains indicate that squalene accumulates at the domain periphery in a manner similar to the accumulation of cholesterol at domain boundaries in their mixtures with phospholipids. Taken together, the results presented here support the notion that squalene plays a role in modulating molecular packing and lateral organization (i.e., domain formation) in the membranes of archaea analogous to that of cholesterol in eukaryotic membranes.

Original languageEnglish (US)
Pages (from-to)7922-7930
Number of pages9
JournalLangmuir
Volume29
Issue number25
DOIs
StatePublished - Jun 25 2013

Fingerprint

Squalene
Lipids
lipids
Monolayers
Compressibility
Cholesterol
Membranes
compressibility
Atomic force microscopy
cholesterol
membranes
Phospholipids
atomic force microscopy
Topography
Isotherms
monomolecular films
Experiments
rims
topography
isotherms

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Gilmore, S. F., Yao, A. I., Tietel, Z., Kind, T., Facciotti, M. T., & Parikh, A. N. (2013). Role of squalene in the organization of monolayers derived from lipid extracts of halobacterium salinarum. Langmuir, 29(25), 7922-7930. https://doi.org/10.1021/la401412t

Role of squalene in the organization of monolayers derived from lipid extracts of halobacterium salinarum. / Gilmore, Sean F.; Yao, Andrew I.; Tietel, Zipora; Kind, Tobias; Facciotti, Marc T.; Parikh, Atul N.

In: Langmuir, Vol. 29, No. 25, 25.06.2013, p. 7922-7930.

Research output: Contribution to journalArticle

Gilmore, SF, Yao, AI, Tietel, Z, Kind, T, Facciotti, MT & Parikh, AN 2013, 'Role of squalene in the organization of monolayers derived from lipid extracts of halobacterium salinarum', Langmuir, vol. 29, no. 25, pp. 7922-7930. https://doi.org/10.1021/la401412t
Gilmore, Sean F. ; Yao, Andrew I. ; Tietel, Zipora ; Kind, Tobias ; Facciotti, Marc T. ; Parikh, Atul N. / Role of squalene in the organization of monolayers derived from lipid extracts of halobacterium salinarum. In: Langmuir. 2013 ; Vol. 29, No. 25. pp. 7922-7930.
@article{1c015155e6ba4685835e5f944ff7dbd3,
title = "Role of squalene in the organization of monolayers derived from lipid extracts of halobacterium salinarum",
abstract = "We have studied interfacial compressibility and lateral organization in monolayer configurations of total (squalene containing) and polar (squalene-devoid) lipid extracts of Halobacterium salinarum NRC-1, an extremely halophilic archaeon. Pressure-area isotherms derived from Langmuir experiments reveal that packing characteristics and elastic compressibility are strongly influenced by the presence of squalene in the total lipid extract. In conjunction with control experiments using mixtures of DPhPC and squalene, our results establish that the presence of squalene significantly extends elastic area compressibility of total lipid extracts, suggesting it has a role in facilitating tighter packing of archaeal lipid mixtures. Moreover, we find that squalene also influences spatial organization in archaeal membranes. Epifluorescence and atomic force microscopy characterization of Langmuir monolayers transferred onto solid hydrophilic substrates reveal an unusual domain morphology. Individual domains of microscopic dimensions (as well as their extended networks) exhibiting a peculiar bowl-like topography are evident in atomic force microscopy images. The tall rims outlining individual domains indicate that squalene accumulates at the domain periphery in a manner similar to the accumulation of cholesterol at domain boundaries in their mixtures with phospholipids. Taken together, the results presented here support the notion that squalene plays a role in modulating molecular packing and lateral organization (i.e., domain formation) in the membranes of archaea analogous to that of cholesterol in eukaryotic membranes.",
author = "Gilmore, {Sean F.} and Yao, {Andrew I.} and Zipora Tietel and Tobias Kind and Facciotti, {Marc T.} and Parikh, {Atul N.}",
year = "2013",
month = "6",
day = "25",
doi = "10.1021/la401412t",
language = "English (US)",
volume = "29",
pages = "7922--7930",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "25",

}

TY - JOUR

T1 - Role of squalene in the organization of monolayers derived from lipid extracts of halobacterium salinarum

AU - Gilmore, Sean F.

AU - Yao, Andrew I.

AU - Tietel, Zipora

AU - Kind, Tobias

AU - Facciotti, Marc T.

AU - Parikh, Atul N.

PY - 2013/6/25

Y1 - 2013/6/25

N2 - We have studied interfacial compressibility and lateral organization in monolayer configurations of total (squalene containing) and polar (squalene-devoid) lipid extracts of Halobacterium salinarum NRC-1, an extremely halophilic archaeon. Pressure-area isotherms derived from Langmuir experiments reveal that packing characteristics and elastic compressibility are strongly influenced by the presence of squalene in the total lipid extract. In conjunction with control experiments using mixtures of DPhPC and squalene, our results establish that the presence of squalene significantly extends elastic area compressibility of total lipid extracts, suggesting it has a role in facilitating tighter packing of archaeal lipid mixtures. Moreover, we find that squalene also influences spatial organization in archaeal membranes. Epifluorescence and atomic force microscopy characterization of Langmuir monolayers transferred onto solid hydrophilic substrates reveal an unusual domain morphology. Individual domains of microscopic dimensions (as well as their extended networks) exhibiting a peculiar bowl-like topography are evident in atomic force microscopy images. The tall rims outlining individual domains indicate that squalene accumulates at the domain periphery in a manner similar to the accumulation of cholesterol at domain boundaries in their mixtures with phospholipids. Taken together, the results presented here support the notion that squalene plays a role in modulating molecular packing and lateral organization (i.e., domain formation) in the membranes of archaea analogous to that of cholesterol in eukaryotic membranes.

AB - We have studied interfacial compressibility and lateral organization in monolayer configurations of total (squalene containing) and polar (squalene-devoid) lipid extracts of Halobacterium salinarum NRC-1, an extremely halophilic archaeon. Pressure-area isotherms derived from Langmuir experiments reveal that packing characteristics and elastic compressibility are strongly influenced by the presence of squalene in the total lipid extract. In conjunction with control experiments using mixtures of DPhPC and squalene, our results establish that the presence of squalene significantly extends elastic area compressibility of total lipid extracts, suggesting it has a role in facilitating tighter packing of archaeal lipid mixtures. Moreover, we find that squalene also influences spatial organization in archaeal membranes. Epifluorescence and atomic force microscopy characterization of Langmuir monolayers transferred onto solid hydrophilic substrates reveal an unusual domain morphology. Individual domains of microscopic dimensions (as well as their extended networks) exhibiting a peculiar bowl-like topography are evident in atomic force microscopy images. The tall rims outlining individual domains indicate that squalene accumulates at the domain periphery in a manner similar to the accumulation of cholesterol at domain boundaries in their mixtures with phospholipids. Taken together, the results presented here support the notion that squalene plays a role in modulating molecular packing and lateral organization (i.e., domain formation) in the membranes of archaea analogous to that of cholesterol in eukaryotic membranes.

UR - http://www.scopus.com/inward/record.url?scp=84879521145&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879521145&partnerID=8YFLogxK

U2 - 10.1021/la401412t

DO - 10.1021/la401412t

M3 - Article

VL - 29

SP - 7922

EP - 7930

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 25

ER -