Role of spi-1 secreted effectors in acute bovine response to salmonella enterica serovar typhimurium: A systems biology analysis approach

Sara D. Lawhon, Sangeeta Khare, Carlos A. Rossetti, Robin E. Everts, Cristi L. Galindo, Sarah A. Luciano, Josely F. Figueiredo, Jairo E S Nunes, Tamara Gull, George S. Davidson, Kenneth L. Drake, Harold R. Garner, Harris A. Lewin, Andreas J Baumler, Leslie Garry Adams

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Salmonella enterica Serovar Typhimurium (S. Typhimurium) causes enterocolitis with diarrhea and polymorphonuclear cell (PMN) influx into the intestinal mucosa in humans and calves. The Salmonella Type III Secretion System (T3SS) encoded at Pathogenicity Island I translocates Salmonella effector proteins SipA, SopA, SopB, SopD, and SopE2 into epithelial cells and is required for induction of diarrhea. These effector proteins act together to induce intestinal fluid secretion and transcription of C-X-C chemokines, recruiting PMNs to the infection site. While individual molecular interactions of the effectors with cultured host cells have been characterized, their combined role in intestinal fluid secretion and inflammation is less understood. We hypothesized that comparison of the bovine intestinal mucosal response to wild type Salmonella and a SipA, SopABDE2 effector mutant relative to uninfected bovine ileum would reveal heretofore unidentified diarrhea-associated host cellular pathways. To determine the coordinated effects of these virulence factors, a bovine ligated ileal loop model was used to measure responses to wild type S. Typhimurium (WT) and a ΔsipA, sopABDE2 mutant (MUT) across 12 hours of infection using a bovine microarray. Data were analyzed using standard microarray analysis and a dynamic Bayesian network modeling approach (DBN). Both analytical methods confirmed increased expression of immune response genes to Salmonella infection and novel gene expression. Gene expression changes mapped to 219 molecular interaction pathways and 1620 gene ontology groups. Bayesian network modeling identified effects of infection on several interrelated signaling pathways including MAPK, Phosphatidylinositol, mTOR, Calcium, Toll-like Receptor, CCR3, Wnt, TGF-β, and Regulation of Actin Cytoskeleton and Apoptosis that were used to model of host-pathogen interactions. Comparison of WT and MUT demonstrated significantly different patterns of host response at early time points of infection (15 minutes, 30 minutes and one hour) within phosphatidylinositol, CCR3, Wnt, and TGF-β signaling pathways and the regulation of actin cytoskeleton pathway.

Original languageEnglish (US)
Article numbere26869
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 11 2011

Fingerprint

Salmonella
Salmonella enterica
Systems Biology
Salmonella Typhimurium
Intestinal Secretions
Fluids and Secretions
type III secretion system
Biological Sciences
Diarrhea
diarrhea
cattle
phosphatidylinositols
Phosphatidylinositols
Infection
microfilaments
Actin Cytoskeleton
infection
Molecular interactions
mutants
Bayesian networks

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lawhon, S. D., Khare, S., Rossetti, C. A., Everts, R. E., Galindo, C. L., Luciano, S. A., ... Adams, L. G. (2011). Role of spi-1 secreted effectors in acute bovine response to salmonella enterica serovar typhimurium: A systems biology analysis approach. PLoS One, 6(11), [e26869]. https://doi.org/10.1371/journal.pone.0026869

Role of spi-1 secreted effectors in acute bovine response to salmonella enterica serovar typhimurium : A systems biology analysis approach. / Lawhon, Sara D.; Khare, Sangeeta; Rossetti, Carlos A.; Everts, Robin E.; Galindo, Cristi L.; Luciano, Sarah A.; Figueiredo, Josely F.; Nunes, Jairo E S; Gull, Tamara; Davidson, George S.; Drake, Kenneth L.; Garner, Harold R.; Lewin, Harris A.; Baumler, Andreas J; Adams, Leslie Garry.

In: PLoS One, Vol. 6, No. 11, e26869, 11.11.2011.

Research output: Contribution to journalArticle

Lawhon, SD, Khare, S, Rossetti, CA, Everts, RE, Galindo, CL, Luciano, SA, Figueiredo, JF, Nunes, JES, Gull, T, Davidson, GS, Drake, KL, Garner, HR, Lewin, HA, Baumler, AJ & Adams, LG 2011, 'Role of spi-1 secreted effectors in acute bovine response to salmonella enterica serovar typhimurium: A systems biology analysis approach', PLoS One, vol. 6, no. 11, e26869. https://doi.org/10.1371/journal.pone.0026869
Lawhon, Sara D. ; Khare, Sangeeta ; Rossetti, Carlos A. ; Everts, Robin E. ; Galindo, Cristi L. ; Luciano, Sarah A. ; Figueiredo, Josely F. ; Nunes, Jairo E S ; Gull, Tamara ; Davidson, George S. ; Drake, Kenneth L. ; Garner, Harold R. ; Lewin, Harris A. ; Baumler, Andreas J ; Adams, Leslie Garry. / Role of spi-1 secreted effectors in acute bovine response to salmonella enterica serovar typhimurium : A systems biology analysis approach. In: PLoS One. 2011 ; Vol. 6, No. 11.
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