Role of galectin-3 in mast cell functions: Galectin-3-deficient mast cells exhibit impaired mediator release and defective JNK expression

Huan Yuan Chen, Bhavya B. Sharma, Lan Yu, Riaz Zuberi, I. Chun Weng, Yuko Kawakami, Toshiaki Kawakami, Daniel K. Hsu, Fu-Tong Liu

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Galectin-3 is a member of the β-galactoside-binding animal lectin family expressed in various cell types, including mast cells. To determine the role of galectin-3 in the function of mast cells, we studied bone marrow-derived mast cells (BMMC) from wild-type (gal3+/+) and galectin-3-deficient (gal3-/-) mice. Cells from the two genotypes showed comparable expression of IgE receptor and c-Kit. However, upon activation by FcεRI cross-linkage, gal3-/- BMMC secreted a significantly lower amount of histamine as well as the cytokine IL-4, compared with gal3+/+ BMMC. In addition, we found significantly reduced passive cutaneous anaphylaxis reactions in gal3-/- mice compared with gal3+/+ mice. These results indicate that there is a defect in the response of mast cells in gal3-/- mice. Unexpectedly, we found that gal3-/- BMMC contained a dramatically lower basal level of JNK1 protein compared with gal3+/+ BMMC, which is probably responsible for the lower EL-4 production. The decreased JNK1 level in gal3-/- BMMC is accompanied by a lower JNK1 mRNA level, suggesting that galectin-3 regulates the transcription of the JNK gene or processing of its RNA. All together, these results point to an important role of galectin-3 in mast cell biology.

Original languageEnglish (US)
Pages (from-to)4991-4997
Number of pages7
JournalJournal of Immunology
Volume177
Issue number8
StatePublished - Oct 15 2006

ASJC Scopus subject areas

  • Immunology

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    Chen, H. Y., Sharma, B. B., Yu, L., Zuberi, R., Weng, I. C., Kawakami, Y., Kawakami, T., Hsu, D. K., & Liu, F-T. (2006). Role of galectin-3 in mast cell functions: Galectin-3-deficient mast cells exhibit impaired mediator release and defective JNK expression. Journal of Immunology, 177(8), 4991-4997.