Abstract
Galectin-3 is a member of the β-galactoside-binding animal lectin family expressed in various cell types, including mast cells. To determine the role of galectin-3 in the function of mast cells, we studied bone marrow-derived mast cells (BMMC) from wild-type (gal3+/+) and galectin-3-deficient (gal3-/-) mice. Cells from the two genotypes showed comparable expression of IgE receptor and c-Kit. However, upon activation by FcεRI cross-linkage, gal3-/- BMMC secreted a significantly lower amount of histamine as well as the cytokine IL-4, compared with gal3+/+ BMMC. In addition, we found significantly reduced passive cutaneous anaphylaxis reactions in gal3-/- mice compared with gal3+/+ mice. These results indicate that there is a defect in the response of mast cells in gal3-/- mice. Unexpectedly, we found that gal3-/- BMMC contained a dramatically lower basal level of JNK1 protein compared with gal3+/+ BMMC, which is probably responsible for the lower EL-4 production. The decreased JNK1 level in gal3-/- BMMC is accompanied by a lower JNK1 mRNA level, suggesting that galectin-3 regulates the transcription of the JNK gene or processing of its RNA. All together, these results point to an important role of galectin-3 in mast cell biology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4991-4997 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 177 |
| Issue number | 8 |
| State | Published - Oct 15 2006 |
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ASJC Scopus subject areas
- Immunology
Cite this
Role of galectin-3 in mast cell functions : Galectin-3-deficient mast cells exhibit impaired mediator release and defective JNK expression. / Chen, Huan Yuan; Sharma, Bhavya B.; Yu, Lan; Zuberi, Riaz; Weng, I. Chun; Kawakami, Yuko; Kawakami, Toshiaki; Hsu, Daniel K.; Liu, Fu-Tong.
In: Journal of Immunology, Vol. 177, No. 8, 15.10.2006, p. 4991-4997.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of galectin-3 in mast cell functions
T2 - Galectin-3-deficient mast cells exhibit impaired mediator release and defective JNK expression
AU - Chen, Huan Yuan
AU - Sharma, Bhavya B.
AU - Yu, Lan
AU - Zuberi, Riaz
AU - Weng, I. Chun
AU - Kawakami, Yuko
AU - Kawakami, Toshiaki
AU - Hsu, Daniel K.
AU - Liu, Fu-Tong
PY - 2006/10/15
Y1 - 2006/10/15
N2 - Galectin-3 is a member of the β-galactoside-binding animal lectin family expressed in various cell types, including mast cells. To determine the role of galectin-3 in the function of mast cells, we studied bone marrow-derived mast cells (BMMC) from wild-type (gal3+/+) and galectin-3-deficient (gal3-/-) mice. Cells from the two genotypes showed comparable expression of IgE receptor and c-Kit. However, upon activation by FcεRI cross-linkage, gal3-/- BMMC secreted a significantly lower amount of histamine as well as the cytokine IL-4, compared with gal3+/+ BMMC. In addition, we found significantly reduced passive cutaneous anaphylaxis reactions in gal3-/- mice compared with gal3+/+ mice. These results indicate that there is a defect in the response of mast cells in gal3-/- mice. Unexpectedly, we found that gal3-/- BMMC contained a dramatically lower basal level of JNK1 protein compared with gal3+/+ BMMC, which is probably responsible for the lower EL-4 production. The decreased JNK1 level in gal3-/- BMMC is accompanied by a lower JNK1 mRNA level, suggesting that galectin-3 regulates the transcription of the JNK gene or processing of its RNA. All together, these results point to an important role of galectin-3 in mast cell biology.
AB - Galectin-3 is a member of the β-galactoside-binding animal lectin family expressed in various cell types, including mast cells. To determine the role of galectin-3 in the function of mast cells, we studied bone marrow-derived mast cells (BMMC) from wild-type (gal3+/+) and galectin-3-deficient (gal3-/-) mice. Cells from the two genotypes showed comparable expression of IgE receptor and c-Kit. However, upon activation by FcεRI cross-linkage, gal3-/- BMMC secreted a significantly lower amount of histamine as well as the cytokine IL-4, compared with gal3+/+ BMMC. In addition, we found significantly reduced passive cutaneous anaphylaxis reactions in gal3-/- mice compared with gal3+/+ mice. These results indicate that there is a defect in the response of mast cells in gal3-/- mice. Unexpectedly, we found that gal3-/- BMMC contained a dramatically lower basal level of JNK1 protein compared with gal3+/+ BMMC, which is probably responsible for the lower EL-4 production. The decreased JNK1 level in gal3-/- BMMC is accompanied by a lower JNK1 mRNA level, suggesting that galectin-3 regulates the transcription of the JNK gene or processing of its RNA. All together, these results point to an important role of galectin-3 in mast cell biology.
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UR - http://www.scopus.com/inward/citedby.url?scp=33749530044&partnerID=8YFLogxK
M3 - Article
C2 - 17015681
AN - SCOPUS:33749530044
VL - 177
SP - 4991
EP - 4997
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 8
ER -