Role of galanin receptor 1 in gastric motility in rat

S. Guerrini, Helen E Raybould, L. Anselmi, A. Agazzi, E. Cervio, J. R. Reeve, M. Tonini, C. Sternini

Research output: Contribution to journalArticle

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Abstract

Galanin actions are mediated by distinct galanin receptors (GAL-R), GAL-R1, -R2 and -R3. We investigated the role of GAL-R1 in gastric motility and the expression of GAL-R1 in the rat stomach. In vivo, in urethane-anaesthetized rats, galanin (equipotent for all GAL-Rs) induced a short inhibition of gastric motility, followed by increase in tonic and phasic gastric motility; the latter was significantly reduced by the GAL-R1 antagonist, RWJ-57408. Galanin 1-16 (high affinity for GAL-R1 and -R2) induced a long-lasting decrease of intragastric pressure, which was not modified by RWJ-57408. In vitro, galanin and galanin 1-16 induced increase of intragastric pressure that was not affected by RWJ-57408. Tetrodotoxin (TTX) did not suppress the galanin excitatory effect, whereas the effect of galanin 1-16 on gastric contraction was increased by TTX- or N-nitro-L-arginine, an inhibitor of nitric oxide synthase. GAL-R1 immunoreactivity was localized to cholinergic and tachykinergic neurons and to neurons immunoreactive for nitric oxide synthase or vasoactive intestinal polypeptide. This study suggests that an extrinsic GAL-R1 pathway mediates the galanin excitatory action, whereas an extrinsic, non GAL-R1 pathway is likely to mediate the galanin inhibitory effect in vivo. GAL-R1 intrinsic neurons do not appear to play a major role in the control of gastric motility.

Original languageEnglish (US)
Pages (from-to)429-438
Number of pages10
JournalNeurogastroenterology and Motility
Volume16
Issue number4
DOIs
StatePublished - Sep 2004

Fingerprint

Receptor, Galanin, Type 1
Galanin
Stomach
Tetrodotoxin
Nitric Oxide Synthase
Galanin Receptors
Neurons
Pressure
Cholinergic Neurons
Vasoactive Intestinal Peptide
Urethane
Arginine

Keywords

  • Enteric pathways
  • Excitatory neurons
  • Extrinsic pathways
  • Inhibitory neurons
  • Intragastric pressure

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Neuroscience(all)

Cite this

Guerrini, S., Raybould, H. E., Anselmi, L., Agazzi, A., Cervio, E., Reeve, J. R., ... Sternini, C. (2004). Role of galanin receptor 1 in gastric motility in rat. Neurogastroenterology and Motility, 16(4), 429-438. https://doi.org/10.1111/j.1365-2982.2004.00534.x

Role of galanin receptor 1 in gastric motility in rat. / Guerrini, S.; Raybould, Helen E; Anselmi, L.; Agazzi, A.; Cervio, E.; Reeve, J. R.; Tonini, M.; Sternini, C.

In: Neurogastroenterology and Motility, Vol. 16, No. 4, 09.2004, p. 429-438.

Research output: Contribution to journalArticle

Guerrini, S, Raybould, HE, Anselmi, L, Agazzi, A, Cervio, E, Reeve, JR, Tonini, M & Sternini, C 2004, 'Role of galanin receptor 1 in gastric motility in rat', Neurogastroenterology and Motility, vol. 16, no. 4, pp. 429-438. https://doi.org/10.1111/j.1365-2982.2004.00534.x
Guerrini, S. ; Raybould, Helen E ; Anselmi, L. ; Agazzi, A. ; Cervio, E. ; Reeve, J. R. ; Tonini, M. ; Sternini, C. / Role of galanin receptor 1 in gastric motility in rat. In: Neurogastroenterology and Motility. 2004 ; Vol. 16, No. 4. pp. 429-438.
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