Role of copper in the regulation and accumulation of superoxide dismutase and metallothionein in rat liver

K. Chung, N. Romero, D. Tinker, Carl L Keen, K. Amemiya, R. Rucker

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Weanling male Sprague-Dawley rats were fed one of four diets that variet in Cu, Zn or Cd content. To the control diet (I) Cu, Zn and Cd were added at 10, 100 and 0 mg/kg diet, respectively. Diets II and III also contained 10 mg/kg of dietary Cu, except that Zn was elevated to 1000 mg/kg for diet II, or Cd was added at 10 mg/kg for diet III. Diet IV was deficient in Cu (<1 mg/kg) with Zn at 100 mg/kg and no added Cd. At wk 6 postweaning, half of the rats fed diets I and IV were injected once with Cd acetate (5 mg Cd/kg body weight). The immediate response to Cd injection was an increase in metallothionein accumulation (three- to fourfold) and in Cu,Zn superoxide dismutase (SOD) accumulation (1.2- to 1.5-fold) in liver. SOD was estimated in an ELISA. These responses were not influenced by a change in Cu status (I vs. IV). However, in functional assays, SOD enzymatic activity was about half that of the control values. In this regard, SOD appears to be given high priority with respect to the utilization of cellular Cu, i.e., a 10-fold reduction in hepatic Cu only resulted in a twofold reduction in SOD activity and the amount of apoenzyme remained at normal levels.

Original languageEnglish (US)
Pages (from-to)859-864
Number of pages6
JournalJournal of Nutrition
Volume118
Issue number7
StatePublished - 1988

Fingerprint

Metallothionein
metallothionein
Superoxide Dismutase
Copper
superoxide dismutase
copper
Diet
liver
Liver
rats
diet
Apoenzymes
apoproteins
weanlings
Sprague Dawley Rats
Acetates
Enzyme-Linked Immunosorbent Assay
acetates
Body Weight
enzyme-linked immunosorbent assay

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Chung, K., Romero, N., Tinker, D., Keen, C. L., Amemiya, K., & Rucker, R. (1988). Role of copper in the regulation and accumulation of superoxide dismutase and metallothionein in rat liver. Journal of Nutrition, 118(7), 859-864.

Role of copper in the regulation and accumulation of superoxide dismutase and metallothionein in rat liver. / Chung, K.; Romero, N.; Tinker, D.; Keen, Carl L; Amemiya, K.; Rucker, R.

In: Journal of Nutrition, Vol. 118, No. 7, 1988, p. 859-864.

Research output: Contribution to journalArticle

Chung, K, Romero, N, Tinker, D, Keen, CL, Amemiya, K & Rucker, R 1988, 'Role of copper in the regulation and accumulation of superoxide dismutase and metallothionein in rat liver', Journal of Nutrition, vol. 118, no. 7, pp. 859-864.
Chung, K. ; Romero, N. ; Tinker, D. ; Keen, Carl L ; Amemiya, K. ; Rucker, R. / Role of copper in the regulation and accumulation of superoxide dismutase and metallothionein in rat liver. In: Journal of Nutrition. 1988 ; Vol. 118, No. 7. pp. 859-864.
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