Role of contraction duration in inducing fast-to-slow contractile and metabolic protein and functional changes in engineered muscle

Alastair Khodabukus, Leslie M. Baehr, Sue C. Bodine, Keith Baar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The role of factors such as frequency, contraction duration and active time in the adaptation to chronic low-frequency electrical stimulation (CLFS) is widely disputed. In this study we explore the ability of contraction duration (0.6, 6, 60, and 600sec) to induce a fast-to-slow shift in engineered muscle while using a stimulation frequency of 10Hz and keeping active time constant at 60%. We found that all contraction durations induced similar slowing of time-to-peak tension. Despite similar increases in total myosin heavy (MHC) levels with stimulation, increasing contraction duration resulted in progressive decreases in total fast myosin. With contraction durations of 60 and 600sec, MHC IIx levels decreased and MHC IIa levels increased. All contraction durations resulted in fast-to-slow shifts in TnT and TnC but increased both fast and slow TnI levels. Half-relaxation slowed to a greater extent with contraction durations of 60 and 600sec despite similar changes in the calcium sequestering proteins calsequestrin and parvalbumin and the calcium uptake protein SERCA. All CLFS groups resulted in greater fatigue resistance than control. Similar increases in GLUT4, mitochondrial enzymes (SDH and ATPsynthase), the fatty acid transporter CPT-1, and the metabolic regulators PGC-1α and MEF2 were found with all contraction durations. However, the mitochondrial enzymes cytochrome C and citrate synthase were increased to greater levels with contraction durations of 60 and 600sec. These results demonstrate that contraction duration plays a pivotal role in dictating the level of CLFS-induced contractile and metabolic adaptations in tissue-engineered skeletal muscle.

Original languageEnglish (US)
Pages (from-to)2489-2497
Number of pages9
JournalJournal of Cellular Physiology
Volume230
Issue number10
DOIs
StatePublished - Oct 1 2015

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Contractile Proteins
Electric Stimulation
Muscle
Myosins
Muscles
Calsequestrin
Calcium
Citrate (si)-Synthase
Parvalbumins
Proteins
Enzymes
Cytochromes
Fatigue
Skeletal Muscle
Fatty Acids
Fatigue of materials
Tissue

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Role of contraction duration in inducing fast-to-slow contractile and metabolic protein and functional changes in engineered muscle. / Khodabukus, Alastair; Baehr, Leslie M.; Bodine, Sue C.; Baar, Keith.

In: Journal of Cellular Physiology, Vol. 230, No. 10, 01.10.2015, p. 2489-2497.

Research output: Contribution to journalArticle

Khodabukus, Alastair ; Baehr, Leslie M. ; Bodine, Sue C. ; Baar, Keith. / Role of contraction duration in inducing fast-to-slow contractile and metabolic protein and functional changes in engineered muscle. In: Journal of Cellular Physiology. 2015 ; Vol. 230, No. 10. pp. 2489-2497.
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