Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and adipose core clock genes in mice and characterization of 24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects

Miguel López-Yoldi, Kimber Stanhope, Marta Garaulet, X. Guoxia Chen, Beatriz Marcos-Gómez, María Paz Carrasco-Benso, Eva M Santa Maria, Xavier Escoté, Vivien Lee, Marinelle V. Nunez, Valentina Medici, Eduardo Martínez-Ansó, Neira Sáinz, Ana E. Huerta, Laura M. Laiglesia, Jesús Prieto, J. Alfredo Martínez, Matilde Bustos, Peter J Havel, Maria J. Moreno-Aliaga

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cardiotrophin (CT)-1 is a regulator of glucose and lipid homeostasis. In the present study, we analyzed whetherCT-1 also acts to peripherally regulate metabolic rhythms andadipose tissue core clock genes inmice. Moreover, the circadian pattern of plasma CT-1 levels was evaluated in normal-weight and overweight subjects. The circadian rhythmicity of oxygen consumption rate (VO2) was disrupted in aged obese CT-1-deficient (CT-1-/-) mice (12 mo). Although circadian rhythms of VO2 were conserved in young lean CT-1-/- mice (2 mo), CT-1 deficiency caused a phase shift of the acrophase. Most of the clock genes studied (Clock, Bmal1, andPer2) displayed a circadian rhythm in adipose tissue of both wild-type (WT) and CT-1-/- mice. However, the pattern was altered in CT-1-/- mice toward a lower percentage of the rhythm or lower amplitude, especially for Bmal1 and Clock.Moreover, CT-1mRNA levels in adipose tissue showed significant circadian fluctuations in young WT mice. In humans, CT-1 plasma profile exhibited a 24-h circadian rhythm in normal-weight but not in overweight subjects. The 24-h pattern of CT-1 was characterized by a pronounced increase during the night (from 02:00 to 08:00). These observations suggest a potential role for CT-1 in the regulation ofmetabolic circadian rhythms.

Original languageEnglish (US)
Pages (from-to)1639-1649
Number of pages11
JournalFASEB Journal
Volume31
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Circadian Rhythm
Clocks
Genes
Weights and Measures
Tissue
Adipose Tissue
cardiotrophin 1
Plasmas
Periodicity
Phase shift
Oxygen Consumption
Homeostasis
Oxygen
Lipids
Glucose

Keywords

  • Adipose tissue
  • Bmal1
  • Cry1
  • Obesity
  • Per2

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and adipose core clock genes in mice and characterization of 24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects. / López-Yoldi, Miguel; Stanhope, Kimber; Garaulet, Marta; Chen, X. Guoxia; Marcos-Gómez, Beatriz; Carrasco-Benso, María Paz; Maria, Eva M Santa; Escoté, Xavier; Lee, Vivien; Nunez, Marinelle V.; Medici, Valentina; Martínez-Ansó, Eduardo; Sáinz, Neira; Huerta, Ana E.; Laiglesia, Laura M.; Prieto, Jesús; Martínez, J. Alfredo; Bustos, Matilde; Havel, Peter J; Moreno-Aliaga, Maria J.

In: FASEB Journal, Vol. 31, No. 4, 01.04.2017, p. 1639-1649.

Research output: Contribution to journalArticle

López-Yoldi, M, Stanhope, K, Garaulet, M, Chen, XG, Marcos-Gómez, B, Carrasco-Benso, MP, Maria, EMS, Escoté, X, Lee, V, Nunez, MV, Medici, V, Martínez-Ansó, E, Sáinz, N, Huerta, AE, Laiglesia, LM, Prieto, J, Martínez, JA, Bustos, M, Havel, PJ & Moreno-Aliaga, MJ 2017, 'Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and adipose core clock genes in mice and characterization of 24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects', FASEB Journal, vol. 31, no. 4, pp. 1639-1649. https://doi.org/10.1096/fj.201600396RR
López-Yoldi, Miguel ; Stanhope, Kimber ; Garaulet, Marta ; Chen, X. Guoxia ; Marcos-Gómez, Beatriz ; Carrasco-Benso, María Paz ; Maria, Eva M Santa ; Escoté, Xavier ; Lee, Vivien ; Nunez, Marinelle V. ; Medici, Valentina ; Martínez-Ansó, Eduardo ; Sáinz, Neira ; Huerta, Ana E. ; Laiglesia, Laura M. ; Prieto, Jesús ; Martínez, J. Alfredo ; Bustos, Matilde ; Havel, Peter J ; Moreno-Aliaga, Maria J. / Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and adipose core clock genes in mice and characterization of 24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects. In: FASEB Journal. 2017 ; Vol. 31, No. 4. pp. 1639-1649.
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AU - Stanhope, Kimber

AU - Garaulet, Marta

AU - Chen, X. Guoxia

AU - Marcos-Gómez, Beatriz

AU - Carrasco-Benso, María Paz

AU - Maria, Eva M Santa

AU - Escoté, Xavier

AU - Lee, Vivien

AU - Nunez, Marinelle V.

AU - Medici, Valentina

AU - Martínez-Ansó, Eduardo

AU - Sáinz, Neira

AU - Huerta, Ana E.

AU - Laiglesia, Laura M.

AU - Prieto, Jesús

AU - Martínez, J. Alfredo

AU - Bustos, Matilde

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