Role of bradykinin in acid-induced mesenteric hyperemia and duodenal villous damage

Felix W. Leung, Fumihiro Iwata, John Kao, Kyoji Seno, Makoto Itoh, Joseph Leung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Intestinal mucosal capsaicin-sensitive afferent nerves mediate, in part, the mesenteric hyperemia after intraduodenal acidification. The hyperemia plays a role in protecting the duodenal mucosa against acid damage. We tested the hypothesis that bradykinin contributes to this protective hyperemia. A specific antagonist of bradykinin will attenuate the hyperemia and exacerbate duodenal villous damage induced by acid. Study 1: Intravenous vehicle, or the specific bradykinin B2 receptor antagonist (HOE 140) was administered to anesthetized rats. This was followed by intraduodenal bolus administration of 160 μM capsaicin or 0.1 N HCl, and then intravenous bradykinin. Study 2: Intravenous administration of vehicle or HOE 140 was followed by duodenal perfusion with 0.1 N HCl. Superior mesenteric artery blood flow (pulsed Doppler flowmetry) (Study 1) and duodenal villous damage (histology) (Study 2) were recorded. HOE 140 significantly reduced the hyperemia induced by bradykinin and intraduodenal capsaicin or acid. Deep villous injury was significantly increased after treatment with HOE 140. These findings support the hypothesis that acid-induced and afferent nerve-mediated mesenteric hyperemia is modulated by a mechanism that involves bradykinin B2 receptor. Antagonism of bradykinin B2 receptor also increased acid-induced deep duodenal villous damage. Thus, maintenance of bradykinin-mediated mesenteric hyperemia, is a previous unrecognized mechanism associated with protection of the rat duodenal mucosa against acid-induced damage.

Original languageEnglish (US)
Pages (from-to)779-790
Number of pages12
JournalLife Sciences
Volume70
Issue number7
DOIs
StatePublished - Jan 4 2002

Fingerprint

Hyperemia
Bradykinin
Acids
Capsaicin
Bradykinin B2 Receptors
Rats
Mucous Membrane
Pulsatile flow
Histology
Acidification
Superior Mesenteric Artery
Rheology
Intravenous Administration
Blood
Perfusion
Maintenance
icatibant
Wounds and Injuries

Keywords

  • Acid-induced duodenal villous injury
  • Afferent nerve-mediated hyperemia
  • Bradykinin
  • Pulsed Doppler flow measurement

ASJC Scopus subject areas

  • Pharmacology

Cite this

Role of bradykinin in acid-induced mesenteric hyperemia and duodenal villous damage. / Leung, Felix W.; Iwata, Fumihiro; Kao, John; Seno, Kyoji; Itoh, Makoto; Leung, Joseph.

In: Life Sciences, Vol. 70, No. 7, 04.01.2002, p. 779-790.

Research output: Contribution to journalArticle

Leung, Felix W. ; Iwata, Fumihiro ; Kao, John ; Seno, Kyoji ; Itoh, Makoto ; Leung, Joseph. / Role of bradykinin in acid-induced mesenteric hyperemia and duodenal villous damage. In: Life Sciences. 2002 ; Vol. 70, No. 7. pp. 779-790.
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