Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations

François Rivier, Ozan Alkan, Alan F. Flint, Kristina Muskiewicz, Paul D. Allen, Philippe Leboulch, Emanuela Gussoni

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The multipotent nature of skeletal muscle-derived side population cells is demonstrated by their myogenic and hematopoietic potential in vivo. However, whether muscle side population cells are derived from the bone marrow is unclear. To study the long-term contribution of the hematopoietic system to muscle side population, whole bone marrow cells from Ly5.1 males or from e-GFP transgenic male mice were transplanted into lethally irradiated Ly5.2 females. Long-term cell trafficking of donor bone marrow cells to muscle side population was monitored 17 times in a 34-week study. Fluorescence-activated cell sorter analyses were used to detect Ly5.1 and GFP+ donor cells, which were confirmed by fluorescence in situ hybridization of the Y-chromosome. Analyses post-transplantation indicated that whereas cells of donor origin could be found in the muscle, donor bone marrow cells had contributed little to the muscle side population. Attempts to increase cell trafficking by induced muscle damage again confirmed that more than 90% of side population cells present in the muscle were derived from the host. These results demonstrate that muscle side population cells are not replenished by the bone marrow and suggest a non-hematopoietic origin for this cell population.

Original languageEnglish (US)
Pages (from-to)1979-1988
Number of pages10
JournalJournal of Cell Science
Volume117
Issue number10
DOIs
StatePublished - Apr 15 2004
Externally publishedYes

Fingerprint

Side-Population Cells
Bone Marrow Cells
Skeletal Muscle
Muscles
Population
Muscle Cells
Bone Marrow
Hematopoietic System
Y Chromosome
Fluorescence In Situ Hybridization
Transgenic Mice
Transplantation
Fluorescence

Keywords

  • Bone marrow transplantation
  • Cell trafficking
  • Fluorescence activated cell sorter (FACS)
  • Muscle side population

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rivier, F., Alkan, O., Flint, A. F., Muskiewicz, K., Allen, P. D., Leboulch, P., & Gussoni, E. (2004). Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations. Journal of Cell Science, 117(10), 1979-1988. https://doi.org/10.1242/jcs.01051

Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations. / Rivier, François; Alkan, Ozan; Flint, Alan F.; Muskiewicz, Kristina; Allen, Paul D.; Leboulch, Philippe; Gussoni, Emanuela.

In: Journal of Cell Science, Vol. 117, No. 10, 15.04.2004, p. 1979-1988.

Research output: Contribution to journalArticle

Rivier, F, Alkan, O, Flint, AF, Muskiewicz, K, Allen, PD, Leboulch, P & Gussoni, E 2004, 'Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations', Journal of Cell Science, vol. 117, no. 10, pp. 1979-1988. https://doi.org/10.1242/jcs.01051
Rivier F, Alkan O, Flint AF, Muskiewicz K, Allen PD, Leboulch P et al. Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations. Journal of Cell Science. 2004 Apr 15;117(10):1979-1988. https://doi.org/10.1242/jcs.01051
Rivier, François ; Alkan, Ozan ; Flint, Alan F. ; Muskiewicz, Kristina ; Allen, Paul D. ; Leboulch, Philippe ; Gussoni, Emanuela. / Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations. In: Journal of Cell Science. 2004 ; Vol. 117, No. 10. pp. 1979-1988.
@article{aac7e5e99af3403cb0d1cddd47496958,
title = "Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations",
abstract = "The multipotent nature of skeletal muscle-derived side population cells is demonstrated by their myogenic and hematopoietic potential in vivo. However, whether muscle side population cells are derived from the bone marrow is unclear. To study the long-term contribution of the hematopoietic system to muscle side population, whole bone marrow cells from Ly5.1 males or from e-GFP transgenic male mice were transplanted into lethally irradiated Ly5.2 females. Long-term cell trafficking of donor bone marrow cells to muscle side population was monitored 17 times in a 34-week study. Fluorescence-activated cell sorter analyses were used to detect Ly5.1 and GFP+ donor cells, which were confirmed by fluorescence in situ hybridization of the Y-chromosome. Analyses post-transplantation indicated that whereas cells of donor origin could be found in the muscle, donor bone marrow cells had contributed little to the muscle side population. Attempts to increase cell trafficking by induced muscle damage again confirmed that more than 90{\%} of side population cells present in the muscle were derived from the host. These results demonstrate that muscle side population cells are not replenished by the bone marrow and suggest a non-hematopoietic origin for this cell population.",
keywords = "Bone marrow transplantation, Cell trafficking, Fluorescence activated cell sorter (FACS), Muscle side population",
author = "Fran{\cc}ois Rivier and Ozan Alkan and Flint, {Alan F.} and Kristina Muskiewicz and Allen, {Paul D.} and Philippe Leboulch and Emanuela Gussoni",
year = "2004",
month = "4",
day = "15",
doi = "10.1242/jcs.01051",
language = "English (US)",
volume = "117",
pages = "1979--1988",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "10",

}

TY - JOUR

T1 - Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations

AU - Rivier, François

AU - Alkan, Ozan

AU - Flint, Alan F.

AU - Muskiewicz, Kristina

AU - Allen, Paul D.

AU - Leboulch, Philippe

AU - Gussoni, Emanuela

PY - 2004/4/15

Y1 - 2004/4/15

N2 - The multipotent nature of skeletal muscle-derived side population cells is demonstrated by their myogenic and hematopoietic potential in vivo. However, whether muscle side population cells are derived from the bone marrow is unclear. To study the long-term contribution of the hematopoietic system to muscle side population, whole bone marrow cells from Ly5.1 males or from e-GFP transgenic male mice were transplanted into lethally irradiated Ly5.2 females. Long-term cell trafficking of donor bone marrow cells to muscle side population was monitored 17 times in a 34-week study. Fluorescence-activated cell sorter analyses were used to detect Ly5.1 and GFP+ donor cells, which were confirmed by fluorescence in situ hybridization of the Y-chromosome. Analyses post-transplantation indicated that whereas cells of donor origin could be found in the muscle, donor bone marrow cells had contributed little to the muscle side population. Attempts to increase cell trafficking by induced muscle damage again confirmed that more than 90% of side population cells present in the muscle were derived from the host. These results demonstrate that muscle side population cells are not replenished by the bone marrow and suggest a non-hematopoietic origin for this cell population.

AB - The multipotent nature of skeletal muscle-derived side population cells is demonstrated by their myogenic and hematopoietic potential in vivo. However, whether muscle side population cells are derived from the bone marrow is unclear. To study the long-term contribution of the hematopoietic system to muscle side population, whole bone marrow cells from Ly5.1 males or from e-GFP transgenic male mice were transplanted into lethally irradiated Ly5.2 females. Long-term cell trafficking of donor bone marrow cells to muscle side population was monitored 17 times in a 34-week study. Fluorescence-activated cell sorter analyses were used to detect Ly5.1 and GFP+ donor cells, which were confirmed by fluorescence in situ hybridization of the Y-chromosome. Analyses post-transplantation indicated that whereas cells of donor origin could be found in the muscle, donor bone marrow cells had contributed little to the muscle side population. Attempts to increase cell trafficking by induced muscle damage again confirmed that more than 90% of side population cells present in the muscle were derived from the host. These results demonstrate that muscle side population cells are not replenished by the bone marrow and suggest a non-hematopoietic origin for this cell population.

KW - Bone marrow transplantation

KW - Cell trafficking

KW - Fluorescence activated cell sorter (FACS)

KW - Muscle side population

UR - http://www.scopus.com/inward/record.url?scp=2942547646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942547646&partnerID=8YFLogxK

U2 - 10.1242/jcs.01051

DO - 10.1242/jcs.01051

M3 - Article

C2 - 15054107

AN - SCOPUS:2942547646

VL - 117

SP - 1979

EP - 1988

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 10

ER -