Role of Aminotransferase IlvE in Production of Branched-Chain Fatty Acids by Lactococcus lactis subsp. lactis

Balasubramanian Ganesan, Bart C Weimer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The objective of this study was to determine the role of a lactococcal branched-chain amino acid amino-transferase gene, ilvE, in the production of branched-chain fatty acids. Lactococcus lactis subsp. lactis LM0230 and an ilvE deletion mutant, JLS450, produced branched-chain fatty acids from amino and α-keto acids at levels above α-keto acid spontaneous degradation and the fatty acids' flavor thresholds. The deletion mutant produced the same amounts of branched-chain fatty acids from precursor amino acids as did the parent. This was not the case, however, for the production of branched-chain fatty acids from the corresponding precursor α-keto acids. The deletion mutant produced a set of fatty acids different from that produced by the parent. We concluded from these observations that ilvE plays a role in the specific type of fatty acids produced but has little influence on the total amount of fatty acids produced by lactococci.

Original languageEnglish (US)
Pages (from-to)638-641
Number of pages4
JournalApplied and Environmental Microbiology
Volume70
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

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branched chain fatty acids
Lactococcus lactis subsp. lactis
Lactococcus lactis
transaminases
Transaminases
keto acids
Fatty Acids
fatty acid
fatty acids
Keto Acids
mutants
Lactococcus
branched chain amino acids
transferases
acid
flavor
amino acid
Amino Acids
Branched Chain Amino Acids
amino acids

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Role of Aminotransferase IlvE in Production of Branched-Chain Fatty Acids by Lactococcus lactis subsp. lactis. / Ganesan, Balasubramanian; Weimer, Bart C.

In: Applied and Environmental Microbiology, Vol. 70, No. 1, 01.2004, p. 638-641.

Research output: Contribution to journalArticle

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