Role of Aminotransferase IlvE in Production of Branched-Chain Fatty Acids by Lactococcus lactis subsp. lactis

Balasubramanian Ganesan, Bart C Weimer

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The objective of this study was to determine the role of a lactococcal branched-chain amino acid amino-transferase gene, ilvE, in the production of branched-chain fatty acids. Lactococcus lactis subsp. lactis LM0230 and an ilvE deletion mutant, JLS450, produced branched-chain fatty acids from amino and α-keto acids at levels above α-keto acid spontaneous degradation and the fatty acids' flavor thresholds. The deletion mutant produced the same amounts of branched-chain fatty acids from precursor amino acids as did the parent. This was not the case, however, for the production of branched-chain fatty acids from the corresponding precursor α-keto acids. The deletion mutant produced a set of fatty acids different from that produced by the parent. We concluded from these observations that ilvE plays a role in the specific type of fatty acids produced but has little influence on the total amount of fatty acids produced by lactococci.

Original languageEnglish (US)
Pages (from-to)638-641
Number of pages4
JournalApplied and Environmental Microbiology
Volume70
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

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