Role of allopregnanolone on cerebellar granule cells neurogenesis

E. A. Keller, A. Zamparini, Laura N Borodinsky, M. C. Gravielle, M. L. Fiszman

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1-1 μM) increased [ 3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl 2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABA A receptors activation.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalDevelopmental Brain Research
Issue number1
StatePublished - Oct 15 2004
Externally publishedYes


  • Allopregnanolone
  • Calcium
  • Cerebellum
  • GABA receptor
  • Neurogenesis
  • Proliferation

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience


Dive into the research topics of 'Role of allopregnanolone on cerebellar granule cells neurogenesis'. Together they form a unique fingerprint.

Cite this