Role of allopregnanolone on cerebellar granule cells neurogenesis

E. A. Keller, A. Zamparini, Laura N Borodinsky, M. C. Gravielle, M. L. Fiszman

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1-1 μM) increased [ 3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl 2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABA A receptors activation.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalDevelopmental Brain Research
Volume153
Issue number1
DOIs
StatePublished - Oct 15 2004
Externally publishedYes

Fingerprint

Pregnanolone
Neurogenesis
Picrotoxin
GABA-A Receptors
Calcium Channels
Nifedipine
Thymidine
Neurotransmitter Agents
Cell Count
Cell Proliferation
Calcium

Keywords

  • Allopregnanolone
  • Calcium
  • Cerebellum
  • GABA receptor
  • Neurogenesis
  • Proliferation

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Role of allopregnanolone on cerebellar granule cells neurogenesis. / Keller, E. A.; Zamparini, A.; Borodinsky, Laura N; Gravielle, M. C.; Fiszman, M. L.

In: Developmental Brain Research, Vol. 153, No. 1, 15.10.2004, p. 13-17.

Research output: Contribution to journalArticle

Keller, E. A. ; Zamparini, A. ; Borodinsky, Laura N ; Gravielle, M. C. ; Fiszman, M. L. / Role of allopregnanolone on cerebellar granule cells neurogenesis. In: Developmental Brain Research. 2004 ; Vol. 153, No. 1. pp. 13-17.
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