Role of allopregnanolone on cerebellar granule cells neurogenesis

E. A. Keller, A. Zamparini, Laura N Borodinsky, M. C. Gravielle, M. L. Fiszman

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1-1 μM) increased [ 3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl 2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABA A receptors activation.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalDevelopmental Brain Research
Volume153
Issue number1
DOIs
StatePublished - Oct 15 2004
Externally publishedYes

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Keywords

  • Allopregnanolone
  • Calcium
  • Cerebellum
  • GABA receptor
  • Neurogenesis
  • Proliferation

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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