Role for the target enzyme in deactivation of photoreceptor G protein in vivo

Stephen H. Tsang, Marie E Burns, Peter D. Calvert, Peter Gouras, Denis A. Baylor, Stephen P. Goff, Vadim Y. Arshavsky

Research output: Contribution to journalArticle

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Abstract

Heterotrimeric guanosine 5'-triphosphate (GTP)-binding proteins (G proteins) are deactivated by hydrolysis of the GTP that they bind when activated by transmembrane receptors. Transducin, the G protein that relays visual excitation from rhodopsin to the cyclic guanosine 3',5'-monophosphate phosphodiesterase (PDE) in retinal photoreceptors, must be deactivated for the light response to recover. A point mutation in the γ subunit of PDE impaired transducin-PDE interactions and slowed the recovery rate of the flash response in transgenic mouse rods. These results indicate that the normal deactivation of transducin in vivo requires the G protein to interact with its target enzyme.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalScience
Volume282
Issue number5386
DOIs
StatePublished - Oct 2 1998
Externally publishedYes

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Transducin
Phosphoric Diester Hydrolases
GTP-Binding Proteins
Guanosine Triphosphate
Enzymes
Vertebrate Photoreceptor Cells
Rhodopsin
Cyclic GMP
Point Mutation
Transgenic Mice
Carrier Proteins
Hydrolysis
Light

ASJC Scopus subject areas

  • General

Cite this

Tsang, S. H., Burns, M. E., Calvert, P. D., Gouras, P., Baylor, D. A., Goff, S. P., & Arshavsky, V. Y. (1998). Role for the target enzyme in deactivation of photoreceptor G protein in vivo. Science, 282(5386), 117-121. https://doi.org/10.1126/science.282.5386.117

Role for the target enzyme in deactivation of photoreceptor G protein in vivo. / Tsang, Stephen H.; Burns, Marie E; Calvert, Peter D.; Gouras, Peter; Baylor, Denis A.; Goff, Stephen P.; Arshavsky, Vadim Y.

In: Science, Vol. 282, No. 5386, 02.10.1998, p. 117-121.

Research output: Contribution to journalArticle

Tsang, SH, Burns, ME, Calvert, PD, Gouras, P, Baylor, DA, Goff, SP & Arshavsky, VY 1998, 'Role for the target enzyme in deactivation of photoreceptor G protein in vivo', Science, vol. 282, no. 5386, pp. 117-121. https://doi.org/10.1126/science.282.5386.117
Tsang SH, Burns ME, Calvert PD, Gouras P, Baylor DA, Goff SP et al. Role for the target enzyme in deactivation of photoreceptor G protein in vivo. Science. 1998 Oct 2;282(5386):117-121. https://doi.org/10.1126/science.282.5386.117
Tsang, Stephen H. ; Burns, Marie E ; Calvert, Peter D. ; Gouras, Peter ; Baylor, Denis A. ; Goff, Stephen P. ; Arshavsky, Vadim Y. / Role for the target enzyme in deactivation of photoreceptor G protein in vivo. In: Science. 1998 ; Vol. 282, No. 5386. pp. 117-121.
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