Abstract
Current genomic studies are limited by the availability of fresh tissue samples. Here, we show that Illumina RNA sequencing of formalin-fixed diagnostic tumor samples produces gene expression that is strongly correlated with matched frozen tumor samples (r>0.89). In addition, sequence variations identified from FFPE RNA show 99.67% concordance with that from exome sequencing of matched frozen tumor samples. Because FFPE is a routine diagnostic sample preparation, the feasibility results reported here will facilitate the setup of large-scale research and clinical studies in medical genomics that are currently limited by the availability of fresh frozen samples.
| Original language | English (US) |
|---|---|
| Article number | 12335 |
| Journal | Scientific reports |
| Volume | 5 |
| DOIs | |
| State | Published - Jul 23 2015 |
| Externally published | Yes |
ASJC Scopus subject areas
- General
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Cite this
Graw, S., Meier, R., Minn, K., Bloomer, C., Godwin, A. K., Fridley, B., Vlad, A., Beyerlein, P., & Chien, J. (2015). Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples. Scientific reports, 5, [12335]. https://doi.org/10.1038/srep12335