RNA transcriptional biosignature analysis for identifying febrile infants with serious bacterial infections in the emergency department: A feasibility study

Febrile Infant Working Group for the Pediatric Emergency Care Applied Research Network (PECARN)

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives: To develop the infrastructure and demonstrate the feasibility of conducting microarray-based RNA transcriptional profile analyses for the diagnosis of serious bacterial infections in febrile infants 60 days and younger in a multicenter pediatric emergency research network. Methods: We designed a prospective multicenter cohort study with the aim of enrolling more than 4000 febrile infants 60 days and younger. To ensure success of conducting complex genomic studies in emergency department (ED) settings, we established an infrastructure within the Pediatric Emergency Care Applied Research Network, including 21 sites, to evaluate RNA transcriptional profiles in young febrile infants. We developed a comprehensive manual of operations and trained site investigators to obtain and process blood samples for RNA extraction and genomic analyses. We created standard operating procedures for blood sample collection, processing, storage, shipping, and analyses. We planned to prospectively identify, enroll, and collect 1 mL blood samples for genomic analyses from eligible patients to identify logistical issues with study procedures. Finally, we planned to batch blood samples and determined RNA quantity and quality at the central microarray laboratory and organized data analysis with the Pediatric Emergency Care Applied Research Network data coordinating center. Below we report on establishment of the infrastructure and the feasibility success in the first year based on the enrollment of a limited number of patients. Results: We successfully established the infrastructure at 21 EDs. Over the first 5 months we enrolled 79% (74 of 94) of eligible febrile infants. We were able to obtain and ship 1 mL of blood from 74% (55 of 74) of enrolled participants, with at least 1 sample per participating ED. The 55 samples were shipped and evaluated at the microarray laboratory, and 95% (52 of 55) of blood samples were of adequate quality and contained sufficient RNA for expression analysis. Conclusions: It is possible to create a robust infrastructure to conduct genomic studies in young febrile infants in the context of a multicenter pediatric ED research setting. The sufficient quantity and high quality of RNA obtained suggests that whole blood transcriptional profile analysis for the diagnostic evaluation of young febrile infants can be successfully performed in this setting.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalPediatric Emergency Care
Volume31
Issue number1
DOIs
StatePublished - Jan 21 2015

Fingerprint

Feasibility Studies
Bacterial Infections
Hospital Emergency Service
Fever
RNA
Pediatrics
Emergency Medical Services
Research
Ships
Multicenter Studies
Emergencies
Cohort Studies
Research Personnel

Keywords

  • febrile infant
  • microarray
  • RNA transcriptional profiles
  • serious bacterial infection

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Emergency Medicine

Cite this

RNA transcriptional biosignature analysis for identifying febrile infants with serious bacterial infections in the emergency department : A feasibility study. / Febrile Infant Working Group for the Pediatric Emergency Care Applied Research Network (PECARN).

In: Pediatric Emergency Care, Vol. 31, No. 1, 21.01.2015, p. 1-5.

Research output: Contribution to journalArticle

Febrile Infant Working Group for the Pediatric Emergency Care Applied Research Network (PECARN). / RNA transcriptional biosignature analysis for identifying febrile infants with serious bacterial infections in the emergency department : A feasibility study. In: Pediatric Emergency Care. 2015 ; Vol. 31, No. 1. pp. 1-5.
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abstract = "Objectives: To develop the infrastructure and demonstrate the feasibility of conducting microarray-based RNA transcriptional profile analyses for the diagnosis of serious bacterial infections in febrile infants 60 days and younger in a multicenter pediatric emergency research network. Methods: We designed a prospective multicenter cohort study with the aim of enrolling more than 4000 febrile infants 60 days and younger. To ensure success of conducting complex genomic studies in emergency department (ED) settings, we established an infrastructure within the Pediatric Emergency Care Applied Research Network, including 21 sites, to evaluate RNA transcriptional profiles in young febrile infants. We developed a comprehensive manual of operations and trained site investigators to obtain and process blood samples for RNA extraction and genomic analyses. We created standard operating procedures for blood sample collection, processing, storage, shipping, and analyses. We planned to prospectively identify, enroll, and collect 1 mL blood samples for genomic analyses from eligible patients to identify logistical issues with study procedures. Finally, we planned to batch blood samples and determined RNA quantity and quality at the central microarray laboratory and organized data analysis with the Pediatric Emergency Care Applied Research Network data coordinating center. Below we report on establishment of the infrastructure and the feasibility success in the first year based on the enrollment of a limited number of patients. Results: We successfully established the infrastructure at 21 EDs. Over the first 5 months we enrolled 79{\%} (74 of 94) of eligible febrile infants. We were able to obtain and ship 1 mL of blood from 74{\%} (55 of 74) of enrolled participants, with at least 1 sample per participating ED. The 55 samples were shipped and evaluated at the microarray laboratory, and 95{\%} (52 of 55) of blood samples were of adequate quality and contained sufficient RNA for expression analysis. Conclusions: It is possible to create a robust infrastructure to conduct genomic studies in young febrile infants in the context of a multicenter pediatric ED research setting. The sufficient quantity and high quality of RNA obtained suggests that whole blood transcriptional profile analysis for the diagnostic evaluation of young febrile infants can be successfully performed in this setting.",
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T2 - A feasibility study

AU - Febrile Infant Working Group for the Pediatric Emergency Care Applied Research Network (PECARN)

AU - Mahajan, Prashant

AU - Kuppermann, Nathan

AU - Suarez, Nicolas

AU - Mejias, Asuncion

AU - Casper, Charlie

AU - Dean, J. Michael

AU - Ramilo, Octavio

AU - Powell, E.

AU - Levine, D.

AU - Tunik, M.

AU - Nigrovic, L.

AU - Roosevelt, G.

AU - Bjaj, L.

AU - Alpern, E.

AU - Browne, L.

AU - Atabaki, S.

AU - Ruddy, R.

AU - Hoyle, J.

AU - Borgialli, D.

AU - Crain, E.

AU - Blumberg, S.

AU - Anders, J.

AU - Bonsu, B.

AU - Cohen, D.

AU - Dayan, P.

AU - Greenberg, R.

AU - Jaffe, D.

AU - Muenzar, J.

AU - Cruz, A.

AU - Tzimenatos, Leah S

AU - Gattu, R.

AU - Rodgers, A.

AU - Brayer, A.

AU - Lillis, K.

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N2 - Objectives: To develop the infrastructure and demonstrate the feasibility of conducting microarray-based RNA transcriptional profile analyses for the diagnosis of serious bacterial infections in febrile infants 60 days and younger in a multicenter pediatric emergency research network. Methods: We designed a prospective multicenter cohort study with the aim of enrolling more than 4000 febrile infants 60 days and younger. To ensure success of conducting complex genomic studies in emergency department (ED) settings, we established an infrastructure within the Pediatric Emergency Care Applied Research Network, including 21 sites, to evaluate RNA transcriptional profiles in young febrile infants. We developed a comprehensive manual of operations and trained site investigators to obtain and process blood samples for RNA extraction and genomic analyses. We created standard operating procedures for blood sample collection, processing, storage, shipping, and analyses. We planned to prospectively identify, enroll, and collect 1 mL blood samples for genomic analyses from eligible patients to identify logistical issues with study procedures. Finally, we planned to batch blood samples and determined RNA quantity and quality at the central microarray laboratory and organized data analysis with the Pediatric Emergency Care Applied Research Network data coordinating center. Below we report on establishment of the infrastructure and the feasibility success in the first year based on the enrollment of a limited number of patients. Results: We successfully established the infrastructure at 21 EDs. Over the first 5 months we enrolled 79% (74 of 94) of eligible febrile infants. We were able to obtain and ship 1 mL of blood from 74% (55 of 74) of enrolled participants, with at least 1 sample per participating ED. The 55 samples were shipped and evaluated at the microarray laboratory, and 95% (52 of 55) of blood samples were of adequate quality and contained sufficient RNA for expression analysis. Conclusions: It is possible to create a robust infrastructure to conduct genomic studies in young febrile infants in the context of a multicenter pediatric ED research setting. The sufficient quantity and high quality of RNA obtained suggests that whole blood transcriptional profile analysis for the diagnostic evaluation of young febrile infants can be successfully performed in this setting.

AB - Objectives: To develop the infrastructure and demonstrate the feasibility of conducting microarray-based RNA transcriptional profile analyses for the diagnosis of serious bacterial infections in febrile infants 60 days and younger in a multicenter pediatric emergency research network. Methods: We designed a prospective multicenter cohort study with the aim of enrolling more than 4000 febrile infants 60 days and younger. To ensure success of conducting complex genomic studies in emergency department (ED) settings, we established an infrastructure within the Pediatric Emergency Care Applied Research Network, including 21 sites, to evaluate RNA transcriptional profiles in young febrile infants. We developed a comprehensive manual of operations and trained site investigators to obtain and process blood samples for RNA extraction and genomic analyses. We created standard operating procedures for blood sample collection, processing, storage, shipping, and analyses. We planned to prospectively identify, enroll, and collect 1 mL blood samples for genomic analyses from eligible patients to identify logistical issues with study procedures. Finally, we planned to batch blood samples and determined RNA quantity and quality at the central microarray laboratory and organized data analysis with the Pediatric Emergency Care Applied Research Network data coordinating center. Below we report on establishment of the infrastructure and the feasibility success in the first year based on the enrollment of a limited number of patients. Results: We successfully established the infrastructure at 21 EDs. Over the first 5 months we enrolled 79% (74 of 94) of eligible febrile infants. We were able to obtain and ship 1 mL of blood from 74% (55 of 74) of enrolled participants, with at least 1 sample per participating ED. The 55 samples were shipped and evaluated at the microarray laboratory, and 95% (52 of 55) of blood samples were of adequate quality and contained sufficient RNA for expression analysis. Conclusions: It is possible to create a robust infrastructure to conduct genomic studies in young febrile infants in the context of a multicenter pediatric ED research setting. The sufficient quantity and high quality of RNA obtained suggests that whole blood transcriptional profile analysis for the diagnostic evaluation of young febrile infants can be successfully performed in this setting.

KW - febrile infant

KW - microarray

KW - RNA transcriptional profiles

KW - serious bacterial infection

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