Abstract
(Figure Presented). We designed an in vitro signal amplification circuit that takes a short RNA input that catalytically activates the Spinach RNA aptamer to produce a fluorescent output. The circuit consists of three RNA strands: an internally blocked Spinach aptamer, a fuel strand, and an input strand (catalyst), as well as the Spinach aptamer ligand 3,5-difluoro-4-hydroxylbenzylidene imidazolinone (DFHBI). The input strand initially displaces the internal inhibitory strand to activate the fluorescent aptamer while exposing a toehold to which the fuel strand can bind to further displace and recycle the input strand. Under a favorable condition, one input strand was able to activate up to five molecules of the internally blocked Spinach aptamer in 185 min at 30 °C. The simple RNA circuit reported here serves as a model for catalytic activation of arbitrary RNA effectors by chemical triggers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 655-658 |
| Number of pages | 4 |
| Journal | ACS Synthetic Biology |
| Volume | 4 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 15 2015 |
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Keywords
- RNA circuit
- RNA engineering
- signal amplification
- Spinach aptamer
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Biomedical Engineering
Cite this
RNA Signal Amplifier Circuit with Integrated Fluorescence Output. / Akter, Farhima; Yokobayashi, Yohei.
In: ACS Synthetic Biology, Vol. 4, No. 5, 15.05.2015, p. 655-658.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RNA Signal Amplifier Circuit with Integrated Fluorescence Output
AU - Akter, Farhima
AU - Yokobayashi, Yohei
PY - 2015/5/15
Y1 - 2015/5/15
N2 - (Figure Presented). We designed an in vitro signal amplification circuit that takes a short RNA input that catalytically activates the Spinach RNA aptamer to produce a fluorescent output. The circuit consists of three RNA strands: an internally blocked Spinach aptamer, a fuel strand, and an input strand (catalyst), as well as the Spinach aptamer ligand 3,5-difluoro-4-hydroxylbenzylidene imidazolinone (DFHBI). The input strand initially displaces the internal inhibitory strand to activate the fluorescent aptamer while exposing a toehold to which the fuel strand can bind to further displace and recycle the input strand. Under a favorable condition, one input strand was able to activate up to five molecules of the internally blocked Spinach aptamer in 185 min at 30 °C. The simple RNA circuit reported here serves as a model for catalytic activation of arbitrary RNA effectors by chemical triggers.
AB - (Figure Presented). We designed an in vitro signal amplification circuit that takes a short RNA input that catalytically activates the Spinach RNA aptamer to produce a fluorescent output. The circuit consists of three RNA strands: an internally blocked Spinach aptamer, a fuel strand, and an input strand (catalyst), as well as the Spinach aptamer ligand 3,5-difluoro-4-hydroxylbenzylidene imidazolinone (DFHBI). The input strand initially displaces the internal inhibitory strand to activate the fluorescent aptamer while exposing a toehold to which the fuel strand can bind to further displace and recycle the input strand. Under a favorable condition, one input strand was able to activate up to five molecules of the internally blocked Spinach aptamer in 185 min at 30 °C. The simple RNA circuit reported here serves as a model for catalytic activation of arbitrary RNA effectors by chemical triggers.
KW - RNA circuit
KW - RNA engineering
KW - signal amplification
KW - Spinach aptamer
UR - http://www.scopus.com/inward/record.url?scp=84929627568&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929627568&partnerID=8YFLogxK
U2 - 10.1021/sb500314r
DO - 10.1021/sb500314r
M3 - Article
C2 - 25354355
AN - SCOPUS:84929627568
VL - 4
SP - 655
EP - 658
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
SN - 2161-5063
IS - 5
ER -