RNA-binding protein RBM24 regulates p63 expression via mRNA stability

Enshun Xu, Jin Zhang, Min Zhang, Yuqian Jiang, Seong Jun Cho, Xinbin Chen

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

p63, a p53 family member, plays pivotal roles in epidermal development, aging, and tumorigenesis. Thus, understanding how p63 expression is controlled has biological and clinical importance. RBM24 is an RNA-binding protein and shares a high sequence similarity with RBM38, a critical regulator of p63. In this study, we investigated whether RBM24 is capable of regulating p63 expression. Indeed, we found that ectopic expression of RBM24 decreased, whereas knockdown of RBM24 increased, the levels of p63 transcript and protein. To explore the underlying mechanism, we found that RBM24 was able to bind to multiple regions in the p63 3′ untranslated region and, subsequently, destabilize p63 transcript. Furthermore, we showed that the 3′ untranslated region in p63 transcript and the RNA-binding domain in RBM24 were required for RBM24 to bind p63 transcript and consequently, inhibit p63 expression. Taken together, our data provide evidence that RBM24 is a novel regulator of p63 via mRNA stability.

Original languageEnglish (US)
Pages (from-to)359-369
Number of pages11
JournalMolecular Cancer Research
Volume12
Issue number3
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Oncology
  • Medicine(all)

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