Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3

Petr Cejka, Jody L. Plank, Csanad Z. Bachrati, Ian D. Hickson, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

A double Holliday junction (dHJ) is a central intermediate of homologous recombination that can be processed to yield crossover or non-crossover recombination products. To preserve genomic integrity, cells possess mechanisms to avoid crossing over. We show that Saccharomyces cerevisiae Sgs1 and Top3 proteins are sufficient to migrate and disentangle a dHJ to produce exclusively non-crossover recombination products, in a reaction termed dissolution. We show that Rmi1 stimulates dHJ dissolution at low Sgs1-Top3 protein concentrations, although it has no effect on the initial rate of Holliday junction (HJ) migration. Rmi1 serves to stimulate DNA decatenation, removing the last linkages between the repaired and template DNA molecules. Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and thereby contributes to genomic integrity.

Original languageEnglish (US)
Pages (from-to)1377-1382
Number of pages6
JournalNature Structural and Molecular Biology
Volume17
Issue number11
DOIs
StatePublished - Nov 2010

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Cruciform DNA
Genetic Recombination
Recombinational DNA Repair
Homologous Recombination
DNA
Saccharomyces cerevisiae
Proteins
Chromosomes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Cejka, P., Plank, J. L., Bachrati, C. Z., Hickson, I. D., & Kowalczykowski, S. C. (2010). Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3. Nature Structural and Molecular Biology, 17(11), 1377-1382. https://doi.org/10.1038/nsmb.1919

Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3. / Cejka, Petr; Plank, Jody L.; Bachrati, Csanad Z.; Hickson, Ian D.; Kowalczykowski, Stephen C.

In: Nature Structural and Molecular Biology, Vol. 17, No. 11, 11.2010, p. 1377-1382.

Research output: Contribution to journalArticle

Cejka, P, Plank, JL, Bachrati, CZ, Hickson, ID & Kowalczykowski, SC 2010, 'Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3', Nature Structural and Molecular Biology, vol. 17, no. 11, pp. 1377-1382. https://doi.org/10.1038/nsmb.1919
Cejka, Petr ; Plank, Jody L. ; Bachrati, Csanad Z. ; Hickson, Ian D. ; Kowalczykowski, Stephen C. / Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3. In: Nature Structural and Molecular Biology. 2010 ; Vol. 17, No. 11. pp. 1377-1382.
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