Rit signaling contributes to interferon-γ-induced dendritic retraction via p38 mitogen-activated protein kinase activation

Douglas A. Andres, Geng Xian Shi, Donald Bruun, Chris Barnhart, Pamela J Lein

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The proinflammatory cytokine interferon-γ (IFNγ) alters neuronal connectivity via selective regressive effects on dendrites but the signaling pathways that mediate this effect are poorly understood. We recently demonstrated that signaling by Rit, a member of the Ras family of GTPases, modulates dendritic growth in primary cultures of sympathetic and hippocampal neurons. In this study, we investigated a role for Rit signaling in IFNγ-induced dendritic retraction. Expression of a dominant negative Rit mutant inhibited IFNγ-induced dendritic retraction in cultured embryonic rat sympathetic and hippocampal neurons. In pheochromacytoma cells and hippocampal neurons, IFNγ caused rapid Rit activation as indicated by increased GTP binding to Rit. Silencing of Rit by RNA interference suppressed IFNγ-elicited activation of p38 MAPK in pheochromacytoma cells, and pharmacological inhibition of p38 MAPK significantly attenuated the dendrite-inhibiting effects of IFNγ in cultured sympathetic and hippocampal neurons without altering signal transducer and activator of transcription 1 activation. These observations identify Rit as a downstream target of IFNγ and suggest that a novel IFNγ-Rit-p38 signaling pathway contributes to dendritic retraction and may, therefore, represent a potential therapeutic target in diseases with a significant neuroinflammatory component.

Original languageEnglish (US)
Pages (from-to)1436-1447
Number of pages12
JournalJournal of Neurochemistry
Volume107
Issue number5
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

p38 Mitogen-Activated Protein Kinases
Interferons
Chemical activation
Neurons
Dendrites
STAT1 Transcription Factor
ras Proteins
GTP Phosphohydrolases
RNA Interference
Guanosine Triphosphate
Transcriptional Activation
Rats
Pharmacology
RNA
Cytokines
Growth

Keywords

  • Dendrite retraction
  • Hippocampal neuron
  • Interferon-γ
  • p38 mitogen-activated protein kinase
  • Rit
  • Sympathetic neuron

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Rit signaling contributes to interferon-γ-induced dendritic retraction via p38 mitogen-activated protein kinase activation. / Andres, Douglas A.; Shi, Geng Xian; Bruun, Donald; Barnhart, Chris; Lein, Pamela J.

In: Journal of Neurochemistry, Vol. 107, No. 5, 12.2008, p. 1436-1447.

Research output: Contribution to journalArticle

Andres, Douglas A. ; Shi, Geng Xian ; Bruun, Donald ; Barnhart, Chris ; Lein, Pamela J. / Rit signaling contributes to interferon-γ-induced dendritic retraction via p38 mitogen-activated protein kinase activation. In: Journal of Neurochemistry. 2008 ; Vol. 107, No. 5. pp. 1436-1447.
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