Rin4 causes hybrid necrosis and race-specific resistance in an interspecific lettuce hybrid

Marieke J W Jeuken, Ningwen W. Zhang, Leah K. McHale, Koen Pelgrom, Erik Den Boer, Pim Lindhout, Richard W Michelmore, Richard G F Visser, Rients E. Niks

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Some inter- and intraspecific crosses may result in reduced viability or sterility in the offspring, often due to genetic incompatibilities resulting from interactions between two or more loci. Hybrid necrosis is a postzygotic genetic incompatibility that is phenotypically manifested as necrotic lesions on the plant. We observed hybrid necrosis in interspecific lettuce (Lactuca sativa and Lactuca saligna) hybrids that correlated with resistance to downy mildew. Segregation analysis revealed a specific allelic combination at two interacting loci to be responsible. The allelic interaction had two consequences: (1) a quantitative temperature-dependent autoimmunity reaction leading to necrotic lesions, lethality, and quantitative resistance to an otherwise virulent race of Bremia lactucae; and (2) a qualitative temperature-independent racespecific resistance to an avirulent race of B. lactucae. We demonstrated by transient expression and silencing experiments that one of the two interacting genes was Rin4. In Arabidopsis thaliana, RIN4 is known to interact with multiple R gene products, and their interactions result in hypersensitive resistance to Pseudomonas syringae. Site-directed mutation studies on the necrosis-eliciting allele of Rin4 in lettuce showed that three residues were critical for hybrid necrosis.

Original languageEnglish (US)
Pages (from-to)3368-3378
Number of pages11
JournalPlant Cell
Volume21
Issue number10
DOIs
StatePublished - Oct 2009

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vertical resistance
Lettuce
lettuce
necrosis
Necrosis
Bremia lactucae
lesions (plant)
Lactuca saligna
vpr Genes
Pseudomonas syringae
loci
autoimmunity
Temperature
downy mildew
Lactuca sativa
Autoimmunity
Arabidopsis
Infertility
temperature
genes

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Jeuken, M. J. W., Zhang, N. W., McHale, L. K., Pelgrom, K., Den Boer, E., Lindhout, P., ... Niks, R. E. (2009). Rin4 causes hybrid necrosis and race-specific resistance in an interspecific lettuce hybrid. Plant Cell, 21(10), 3368-3378. https://doi.org/10.1105/tpc.109.070334

Rin4 causes hybrid necrosis and race-specific resistance in an interspecific lettuce hybrid. / Jeuken, Marieke J W; Zhang, Ningwen W.; McHale, Leah K.; Pelgrom, Koen; Den Boer, Erik; Lindhout, Pim; Michelmore, Richard W; Visser, Richard G F; Niks, Rients E.

In: Plant Cell, Vol. 21, No. 10, 10.2009, p. 3368-3378.

Research output: Contribution to journalArticle

Jeuken, MJW, Zhang, NW, McHale, LK, Pelgrom, K, Den Boer, E, Lindhout, P, Michelmore, RW, Visser, RGF & Niks, RE 2009, 'Rin4 causes hybrid necrosis and race-specific resistance in an interspecific lettuce hybrid', Plant Cell, vol. 21, no. 10, pp. 3368-3378. https://doi.org/10.1105/tpc.109.070334
Jeuken MJW, Zhang NW, McHale LK, Pelgrom K, Den Boer E, Lindhout P et al. Rin4 causes hybrid necrosis and race-specific resistance in an interspecific lettuce hybrid. Plant Cell. 2009 Oct;21(10):3368-3378. https://doi.org/10.1105/tpc.109.070334
Jeuken, Marieke J W ; Zhang, Ningwen W. ; McHale, Leah K. ; Pelgrom, Koen ; Den Boer, Erik ; Lindhout, Pim ; Michelmore, Richard W ; Visser, Richard G F ; Niks, Rients E. / Rin4 causes hybrid necrosis and race-specific resistance in an interspecific lettuce hybrid. In: Plant Cell. 2009 ; Vol. 21, No. 10. pp. 3368-3378.
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