RiboTALE: A modular, inducible system for accurate gene expression control

Navneet Rai, Aura Ferreiro, Alexander Neckelmann, Amy Soon, Andrew Yao, Justin Siegel, Marc T. Facciotti, Ilias Tagkopoulos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A limiting factor in synthetic gene circuit design is the number of independent control elements that can be combined together in a single system. Here, we present RiboTALEs, a new class of inducible repressors that combine the specificity of TALEs with the ability of riboswitches to recognize exogenous signals and differentially control protein abundance. We demonstrate the capacity of RiboTALEs, constructed through different combinations of TALE proteins and riboswitches, to rapidly and reproducibly control the expression of downstream targets with a dynamic range of 243.7 ± 17.6-fold, which is adequate for many biotechnological applications.

Original languageEnglish (US)
Article number10658
JournalScientific Reports
Volume5
DOIs
StatePublished - May 29 2015

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Riboswitch
Synthetic Genes
Gene Expression
Gene Regulatory Networks
Proteins

ASJC Scopus subject areas

  • General

Cite this

Rai, N., Ferreiro, A., Neckelmann, A., Soon, A., Yao, A., Siegel, J., ... Tagkopoulos, I. (2015). RiboTALE: A modular, inducible system for accurate gene expression control. Scientific Reports, 5, [10658]. https://doi.org/10.1038/srep10658

RiboTALE : A modular, inducible system for accurate gene expression control. / Rai, Navneet; Ferreiro, Aura; Neckelmann, Alexander; Soon, Amy; Yao, Andrew; Siegel, Justin; Facciotti, Marc T.; Tagkopoulos, Ilias.

In: Scientific Reports, Vol. 5, 10658, 29.05.2015.

Research output: Contribution to journalArticle

Rai, N, Ferreiro, A, Neckelmann, A, Soon, A, Yao, A, Siegel, J, Facciotti, MT & Tagkopoulos, I 2015, 'RiboTALE: A modular, inducible system for accurate gene expression control', Scientific Reports, vol. 5, 10658. https://doi.org/10.1038/srep10658
Rai, Navneet ; Ferreiro, Aura ; Neckelmann, Alexander ; Soon, Amy ; Yao, Andrew ; Siegel, Justin ; Facciotti, Marc T. ; Tagkopoulos, Ilias. / RiboTALE : A modular, inducible system for accurate gene expression control. In: Scientific Reports. 2015 ; Vol. 5.
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