Ribosomal protein L24 defect in belly spot and tail (Bst), a mouse Minute

Edward R. Oliver, Thomas L. Saunders, Susan A. Tarlé, Thomas M Glaser

Research output: Contribution to journalArticlepeer-review

204 Scopus citations

Abstract

Ribosomal protein mutations, termed Minutes, have been instrumental in studying the coordination of cell and tissue growth in Drosophila. Although abundant in flies, equivalent defects in mammals are relatively unknown. Belly spot and tail (Bst) is a semidominant mouse mutation that disrupts pigmentation, somitogenesis and retinal cell fate determination. Here, we identify Bst as a deletion within the Rpl24 riboprotein gene. Bst significantly impairs Rpl24 splicing and ribosome biogenesis. Bst/+ cells have decreased rates of protein synthesis and proliferation, and are outcompeted by wild-type cells in C57BLKS↔ ROSA26 chimeras. Bacterial artificial chromosome (BAC) and cDNA transgenes correct the mutant phenotypes. Our findings establish Bst as a mouse Minute and provide the first detailed characterization of a mammalian ribosomal protein mutation.

Original languageEnglish (US)
Pages (from-to)3907-3920
Number of pages14
JournalDevelopment
Volume131
Issue number16
DOIs
StatePublished - Aug 2004
Externally publishedYes

Keywords

  • Bst
  • Cell cycle
  • Genetics
  • Minute
  • Mouse
  • Retina
  • Ribosome

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Fingerprint Dive into the research topics of 'Ribosomal protein L24 defect in belly spot and tail (Bst), a mouse Minute'. Together they form a unique fingerprint.

Cite this