Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates

Patricia A Conrad, J. W. Thomford, A. Marsh, S. R. Telford, J. F. Anderson, A. Spielman, E. A. Sabin, I. Yamane, D. H. Persing

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The objective of this study was to determine whether different isolates of Babesia microti could be distinguished from morphologically similar isolates of B. gibsoni by using a ribosomal DNA (rDNA) probe. A Babesia-specific rDNA probe was obtained by polymerase chain reaction amplification of sequences from B. microti DNA using universal primers directed against highly conserved portions of the eukaryotic 16S-like rRNA gene. The chemiluminescent rDNA probe hybridized to Southern blots of restriction endonuclease-digested DNA preparations of different isolates of B. gibsoni from infected dogs and B. microti from infected humans and white-footed mice. Restriction fragment length polymorphisms served to differentiate these species. Although the hybridization patterns seen with DNAs from six B. microti isolates did not vary, those of the five B. gibsoni isolates did indicate genotypic variation. We concluded that isolates of B. microti and B. gibsoni can be differentiated on the basis of restriction fragment length polymorphism detected with a chemiluminescent rDNA probe.

Original languageEnglish (US)
Pages (from-to)1210-1215
Number of pages6
JournalJournal of Clinical Microbiology
Volume30
Issue number5
StatePublished - 1992

Fingerprint

Babesia microti
DNA Probes
Ribosomal DNA
Restriction Fragment Length Polymorphisms
DNA
Peromyscus
Babesia
DNA Restriction Enzymes
Southern Blotting
rRNA Genes
Dogs
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Conrad, P. A., Thomford, J. W., Marsh, A., Telford, S. R., Anderson, J. F., Spielman, A., ... Persing, D. H. (1992). Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates. Journal of Clinical Microbiology, 30(5), 1210-1215.

Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates. / Conrad, Patricia A; Thomford, J. W.; Marsh, A.; Telford, S. R.; Anderson, J. F.; Spielman, A.; Sabin, E. A.; Yamane, I.; Persing, D. H.

In: Journal of Clinical Microbiology, Vol. 30, No. 5, 1992, p. 1210-1215.

Research output: Contribution to journalArticle

Conrad, PA, Thomford, JW, Marsh, A, Telford, SR, Anderson, JF, Spielman, A, Sabin, EA, Yamane, I & Persing, DH 1992, 'Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates', Journal of Clinical Microbiology, vol. 30, no. 5, pp. 1210-1215.
Conrad PA, Thomford JW, Marsh A, Telford SR, Anderson JF, Spielman A et al. Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates. Journal of Clinical Microbiology. 1992;30(5):1210-1215.
Conrad, Patricia A ; Thomford, J. W. ; Marsh, A. ; Telford, S. R. ; Anderson, J. F. ; Spielman, A. ; Sabin, E. A. ; Yamane, I. ; Persing, D. H. / Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates. In: Journal of Clinical Microbiology. 1992 ; Vol. 30, No. 5. pp. 1210-1215.
@article{42a8ccb47ca142f992c18eebab6a241d,
title = "Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates",
abstract = "The objective of this study was to determine whether different isolates of Babesia microti could be distinguished from morphologically similar isolates of B. gibsoni by using a ribosomal DNA (rDNA) probe. A Babesia-specific rDNA probe was obtained by polymerase chain reaction amplification of sequences from B. microti DNA using universal primers directed against highly conserved portions of the eukaryotic 16S-like rRNA gene. The chemiluminescent rDNA probe hybridized to Southern blots of restriction endonuclease-digested DNA preparations of different isolates of B. gibsoni from infected dogs and B. microti from infected humans and white-footed mice. Restriction fragment length polymorphisms served to differentiate these species. Although the hybridization patterns seen with DNAs from six B. microti isolates did not vary, those of the five B. gibsoni isolates did indicate genotypic variation. We concluded that isolates of B. microti and B. gibsoni can be differentiated on the basis of restriction fragment length polymorphism detected with a chemiluminescent rDNA probe.",
author = "Conrad, {Patricia A} and Thomford, {J. W.} and A. Marsh and Telford, {S. R.} and Anderson, {J. F.} and A. Spielman and Sabin, {E. A.} and I. Yamane and Persing, {D. H.}",
year = "1992",
language = "English (US)",
volume = "30",
pages = "1210--1215",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Ribosomal DNA probe for differentiation of Babesia microti and B. gibsoni isolates

AU - Conrad, Patricia A

AU - Thomford, J. W.

AU - Marsh, A.

AU - Telford, S. R.

AU - Anderson, J. F.

AU - Spielman, A.

AU - Sabin, E. A.

AU - Yamane, I.

AU - Persing, D. H.

PY - 1992

Y1 - 1992

N2 - The objective of this study was to determine whether different isolates of Babesia microti could be distinguished from morphologically similar isolates of B. gibsoni by using a ribosomal DNA (rDNA) probe. A Babesia-specific rDNA probe was obtained by polymerase chain reaction amplification of sequences from B. microti DNA using universal primers directed against highly conserved portions of the eukaryotic 16S-like rRNA gene. The chemiluminescent rDNA probe hybridized to Southern blots of restriction endonuclease-digested DNA preparations of different isolates of B. gibsoni from infected dogs and B. microti from infected humans and white-footed mice. Restriction fragment length polymorphisms served to differentiate these species. Although the hybridization patterns seen with DNAs from six B. microti isolates did not vary, those of the five B. gibsoni isolates did indicate genotypic variation. We concluded that isolates of B. microti and B. gibsoni can be differentiated on the basis of restriction fragment length polymorphism detected with a chemiluminescent rDNA probe.

AB - The objective of this study was to determine whether different isolates of Babesia microti could be distinguished from morphologically similar isolates of B. gibsoni by using a ribosomal DNA (rDNA) probe. A Babesia-specific rDNA probe was obtained by polymerase chain reaction amplification of sequences from B. microti DNA using universal primers directed against highly conserved portions of the eukaryotic 16S-like rRNA gene. The chemiluminescent rDNA probe hybridized to Southern blots of restriction endonuclease-digested DNA preparations of different isolates of B. gibsoni from infected dogs and B. microti from infected humans and white-footed mice. Restriction fragment length polymorphisms served to differentiate these species. Although the hybridization patterns seen with DNAs from six B. microti isolates did not vary, those of the five B. gibsoni isolates did indicate genotypic variation. We concluded that isolates of B. microti and B. gibsoni can be differentiated on the basis of restriction fragment length polymorphism detected with a chemiluminescent rDNA probe.

UR - http://www.scopus.com/inward/record.url?scp=0026537791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026537791&partnerID=8YFLogxK

M3 - Article

C2 - 1349901

AN - SCOPUS:0026537791

VL - 30

SP - 1210

EP - 1215

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 5

ER -