Rhythmicity of engraftment and altered cell cycle kinetics of cytokine-cultured murine marrow in simulated microgravity compared with static cultures

Gerald A. Colvin, Jean François Lambert, Jane E. Carlson, Christina I. McAuliffe, Mehrdad Abedi, Peter J. Quesenberry

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Space flight with associated microgravity is complicated by "astronaut's anemia" and other hematologic abnormalities. Altered erythroid differentiation, red cell survival, plasma volume, and progenitor numbers have been reported. We studied the impact of microgravity on engraftable stem cells, culturing marrow cells in rotary wall vessel (RWV) culture chambers mimicking microgravity and in normal gravity nonadherent Teflon bottles. A quantitative competitive engraftment technique was assessed under both conditions in lethally irradiated hosts. We assessed 8-wk engraftable stem cells over a period spanning at least one cell cycle for cytokine (FLT-3 ligand, thrombopoietin [TPO], steel factor)-activated marrow stem cells. Engraftable stem cells were supported out to 56 h under microgravity conditions, and this support was superior to that seen in normal-gravity Teflon bottle cultures out to 40 h, with Teflon bottle culture support superior to RWV from 40 to 56 h. A nadir of stem cell number was seen at 40 h in Teflon and 48 h in RWV, suggesting altered marrow stem cell cycle kinetics under microgravity. This is the first study of engraftable stem cells under microgravity conditions, and the differences between microgravity and normal gravity cultures may present opportunities for unique future stem cell expansion strategies.

Original languageEnglish (US)
Pages (from-to)343-351
Number of pages9
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume38
Issue number6
DOIs
StatePublished - Jun 2002
Externally publishedYes

Keywords

  • Bone marrow transplantation
  • Hematopoiesis
  • Microgravity bioreactor
  • Rotating wall vessel
  • RWV
  • Slow turning lateral vessel
  • STLV

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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