Rho GTPases and the downstream effectors actin-related protein 2/3 (Arp2/3) complex and myosin ii induce membrane fusion at self-contacts

Grant M. Sumida, Soichiro Yamada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Actin regulation is required for membrane activities that drive cell adhesion and migration. The Rho GTPase family plays critical roles in actin and membrane dynamics; however, the roles of the Rho GTPase family are not limited to cell adhesion and migration. Using micron-sized obstacles to induce the formation of self-contacts in epithelial cells, we previously showed that self-adhesion is distinct from cell-to-cell adhesion in that self-contacts are eliminated by membrane fusion. In the current study, we identified Rho GTPases, RhoA, Rac1, and Cdc42, as potential upstream regulators of membrane fusion. The RhoA downstream effector myosin II is required for fusion as the expression of mutant myosin light chain reduced membrane fusion. Furthermore, an inhibitor of the Arp2/3 complex, a downstream effector of Rac1 and Cdc42, also reduced self-contact-induced membrane fusion. At self-contacts, while the concentration of E-cadherin diminished, the intensity of GFP-tagged Arp3 rapidly fluctuated then decreased and stabilized after membrane fusion. Taken together, these data suggest that the Arp2/3 complex-mediated actin polymerization brings two opposing membranes into close apposition by possibly excluding E-cadherin from contact sites, thus promoting membrane fusion at self-contacts.

Original languageEnglish (US)
Pages (from-to)3238-3247
Number of pages10
JournalJournal of Biological Chemistry
Volume290
Issue number6
DOIs
StatePublished - Feb 6 2015

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Actin-Related Protein 2-3 Complex
rho GTP-Binding Proteins
Membrane Fusion
Myosins
Fusion reactions
Membranes
Cell Adhesion
Actins
Cell adhesion
Cadherins
Cell Movement
Myosin Type II
Myosin Light Chains
Polymerization
Epithelial Cells
Adhesion

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Rho GTPases and the downstream effectors actin-related protein 2/3 (Arp2/3) complex and myosin ii induce membrane fusion at self-contacts. / Sumida, Grant M.; Yamada, Soichiro.

In: Journal of Biological Chemistry, Vol. 290, No. 6, 06.02.2015, p. 3238-3247.

Research output: Contribution to journalArticle

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