Abstract
Background and Purpose: Development of the rhesus monkey model of Helicobacter pylori has been hampered by problems with serodetection and by the difficulty of identifying specific-pathogen (Helicobacter)-free animals. Our purpose was to determine whether detection could be improved and to determine if pathogen-free monkeys could be derived by nursery rearing. Methods: An enzyme-linked immunoabsorbent assay (ELISA) and a [ 14C]urea breath test were compared to endoscopy to determine H. pylori infection status in rhesus macaques; 18 animals were hand raised in the nursery to determine whether pathogen-free animals could be selected. Results: Helicobacter pylori infection was common in colony-raised young rhesus monkeys and was nearly universal by adulthood. Serodetection, using antigen from rhesus-derived H. pylori strains, was 95% sensitive and 94% specific. The [ 14C]urea breath test was 96% sensitive and 88% specific for detection of chronic Helicobacter infection in rhesus monkeys. Segregation of newborn animals within the first 24 h of life was a reliable method to obtain pathogen-free rhesus monkeys. Conclusion: Isolation of specific-pathogen- free animals, together with better detection methods, may improve the value of the rhesus monkey model for the study of H. pylori pathogenesis, immune response, and vaccine development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 197-201 |
| Number of pages | 5 |
| Journal | Laboratory Animal Science |
| Volume | 49 |
| Issue number | 2 |
| State | Published - 1999 |
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ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
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Rhesus monkey (Macaca mulatta) model of Helicobacter pylori : Noninvasive detection and derivation of specific-pathogen-free monkeys. / Solnick, Jay V; Canfield, Don R.; Yang, Shufang; Parsonnet, Julie.
In: Laboratory Animal Science, Vol. 49, No. 2, 1999, p. 197-201.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rhesus monkey (Macaca mulatta) model of Helicobacter pylori
T2 - Noninvasive detection and derivation of specific-pathogen-free monkeys
AU - Solnick, Jay V
AU - Canfield, Don R.
AU - Yang, Shufang
AU - Parsonnet, Julie
PY - 1999
Y1 - 1999
N2 - Background and Purpose: Development of the rhesus monkey model of Helicobacter pylori has been hampered by problems with serodetection and by the difficulty of identifying specific-pathogen (Helicobacter)-free animals. Our purpose was to determine whether detection could be improved and to determine if pathogen-free monkeys could be derived by nursery rearing. Methods: An enzyme-linked immunoabsorbent assay (ELISA) and a [ 14C]urea breath test were compared to endoscopy to determine H. pylori infection status in rhesus macaques; 18 animals were hand raised in the nursery to determine whether pathogen-free animals could be selected. Results: Helicobacter pylori infection was common in colony-raised young rhesus monkeys and was nearly universal by adulthood. Serodetection, using antigen from rhesus-derived H. pylori strains, was 95% sensitive and 94% specific. The [ 14C]urea breath test was 96% sensitive and 88% specific for detection of chronic Helicobacter infection in rhesus monkeys. Segregation of newborn animals within the first 24 h of life was a reliable method to obtain pathogen-free rhesus monkeys. Conclusion: Isolation of specific-pathogen- free animals, together with better detection methods, may improve the value of the rhesus monkey model for the study of H. pylori pathogenesis, immune response, and vaccine development.
AB - Background and Purpose: Development of the rhesus monkey model of Helicobacter pylori has been hampered by problems with serodetection and by the difficulty of identifying specific-pathogen (Helicobacter)-free animals. Our purpose was to determine whether detection could be improved and to determine if pathogen-free monkeys could be derived by nursery rearing. Methods: An enzyme-linked immunoabsorbent assay (ELISA) and a [ 14C]urea breath test were compared to endoscopy to determine H. pylori infection status in rhesus macaques; 18 animals were hand raised in the nursery to determine whether pathogen-free animals could be selected. Results: Helicobacter pylori infection was common in colony-raised young rhesus monkeys and was nearly universal by adulthood. Serodetection, using antigen from rhesus-derived H. pylori strains, was 95% sensitive and 94% specific. The [ 14C]urea breath test was 96% sensitive and 88% specific for detection of chronic Helicobacter infection in rhesus monkeys. Segregation of newborn animals within the first 24 h of life was a reliable method to obtain pathogen-free rhesus monkeys. Conclusion: Isolation of specific-pathogen- free animals, together with better detection methods, may improve the value of the rhesus monkey model for the study of H. pylori pathogenesis, immune response, and vaccine development.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032916669&partnerID=8YFLogxK
M3 - Article
C2 - 10331550
AN - SCOPUS:0032916669
VL - 49
SP - 197
EP - 201
JO - Comparative Medicine
JF - Comparative Medicine
SN - 1532-0820
IS - 2
ER -