Rhesus macaques previously infected with simian/human immunodeficiency virus are protected from vaginal challenge with pathogenic SIVmac239

Chris J Miller, Michael B. McChesney, Xusheng Lü, Peter J. Dailey, Christine Chutkowski, Ding Lu, Paul Brosio, Bryan Roberts, Yichen Lu

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Abstract

Nontraumatic vaginal inoculation of rhesus macaques with a simian/human immunodeficiency virus (SIV/HIV) chimera containing the envelope gene from HIV-1 89.6 (SHIV 89.6) results in systemic infection (Y. Lu, B. Brosio, M. Lafaile, J. Li, R. G. Collman, J. Sodroski, and C. J. Miller, J. Virol. 70:3045-3050, 1996). A total of five rhesus macaques have each been infected by exposure to at least three intravaginal inoculations of SHIV 89.6. The SHIV 89.6 infection is characterized by a transient viremia that evokes humoral and cellular immune responses to HIV and SIV antigens, but disease does not develop in animals infected with SHIV 89.0. To determine if a previous infection with SHIV 89.6 by vaginal inoculation could protect animals from vaginal challenge with pathogenic SIV, all five animals were intravaginally inoculated twice with pathogenic SIV-mac239. After challenge, all of the SHIV-immunized animals had low or undetectable viral RNA levels in plasma compared to control animals. Three of the five of the SHIV-immunized animals remained virus isolation negative for more than 8 months, while two became virus isolation positive. The presence of SIV Gag-specific cytotoxic T lymphocytes in peripheral blood mononuclear cells and SIV-specific antibodies in cervicovaginal secretions at the time of challenge was associated with resistance to pathogenic SIV infection after vaginal challenge. These results suggest that protection from sexual transmission of HIV may be possible by effectively stimulating both humoral and cellular antiviral immunity in the systemic and genital mucosal immune compartments.

Original languageEnglish (US)
Pages (from-to)1911-1921
Number of pages11
JournalJournal of Virology
Volume71
Issue number3
StatePublished - Mar 1997

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Simian Immunodeficiency Virus
Human immunodeficiency virus
Macaca mulatta
HIV
vaccination
animals
Infection
Cellular Immunity
infection
HIV Antigens
Viruses
cytotoxic T-lymphocytes
chimerism
Viremia
viremia
Viral RNA
Cytotoxic T-Lymphocytes
Humoral Immunity
mononuclear leukocytes
Human immunodeficiency virus 1

ASJC Scopus subject areas

  • Immunology

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Rhesus macaques previously infected with simian/human immunodeficiency virus are protected from vaginal challenge with pathogenic SIVmac239. / Miller, Chris J; McChesney, Michael B.; Lü, Xusheng; Dailey, Peter J.; Chutkowski, Christine; Lu, Ding; Brosio, Paul; Roberts, Bryan; Lu, Yichen.

In: Journal of Virology, Vol. 71, No. 3, 03.1997, p. 1911-1921.

Research output: Contribution to journalArticle

Miller, CJ, McChesney, MB, Lü, X, Dailey, PJ, Chutkowski, C, Lu, D, Brosio, P, Roberts, B & Lu, Y 1997, 'Rhesus macaques previously infected with simian/human immunodeficiency virus are protected from vaginal challenge with pathogenic SIVmac239', Journal of Virology, vol. 71, no. 3, pp. 1911-1921.
Miller, Chris J ; McChesney, Michael B. ; Lü, Xusheng ; Dailey, Peter J. ; Chutkowski, Christine ; Lu, Ding ; Brosio, Paul ; Roberts, Bryan ; Lu, Yichen. / Rhesus macaques previously infected with simian/human immunodeficiency virus are protected from vaginal challenge with pathogenic SIVmac239. In: Journal of Virology. 1997 ; Vol. 71, No. 3. pp. 1911-1921.
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