Rhesus macaque polyclonal and monoclonal antibodies inhibit simian immunodeficiency virus in the presence of human or autologous rhesus effector cells

Donald N. Forthal, Gary Landucci, Kelly Stefano Cole, Marta Marthas, Juan C. Becerra, Koen Van Rompay

Research output: Contribution to journalArticle

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Abstract

Although antibodies can prevent or modulate lentivirus infections in nonhurnan primates, the biological functions of antibody responsible for such effects are not known. We sought to determine the role of antibody-dependent cell-mediated virus inhibition (ADCVI), an antibody function that inhibits virus yield from infected cells in the presence of Fc receptor-bearing effector cells, in preventing or controlling SIVmac251 infection in rhesus macaques (Macaca mulatta). Using CEMx174 cells infected with simian immunodeficiency virus mac251 (SIVmac251), both polyclonal and monoclonal anti-SIV antibodies were capable of potent virus inhibition in the presence of human peripheral blood mononuclear cell (PBMC) effector cells. In the absence of effector cells, virus inhibition was generally very poor. PBMCs from healthy rhesus macaques were also capable of mediating virus inhibition either against SIVmac251-infected CEMx174 cells or against infected, autologous rhesus target cells. We identified both CD14+ cells and, to a lesser extent, CD8+ cells as the effector cell population in the rhesus PBMCs. Finally, pooled, nonneutralizing SIV-antibody-positive serum, shown in a previous study to prevent infection of neonatal macaques after oral SIVmac251 challenge, had potent virus-inhibitory activity in the presence of elector cells; intact immunoglobulin G, rather than F(ab')2, was required for such activity. This is the first demonstration of both humoral and cellular ADCVI functions in the macaque-SIV model. ADCVI activity in nonneutralizing serum that prevents SIV infection suggests that ADCVI may be a protective immune function. Finally, our data underscore the potential importance of Fc-Fc receptor interactions in mediating biological activities of antibody.

Original languageEnglish (US)
Pages (from-to)9217-9225
Number of pages9
JournalJournal of Virology
Volume80
Issue number18
DOIs
StatePublished - Sep 1 2006

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca mulatta
polyclonal antibodies
monoclonal antibodies
Monoclonal Antibodies
Viruses
antibodies
viruses
cells
Antibodies
mononuclear leukocytes
Fc Receptors
Macaca
infection
Lentivirus Infections
Lentivirus
receptors
immunoglobulin G
Virus Diseases

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Rhesus macaque polyclonal and monoclonal antibodies inhibit simian immunodeficiency virus in the presence of human or autologous rhesus effector cells. / Forthal, Donald N.; Landucci, Gary; Cole, Kelly Stefano; Marthas, Marta; Becerra, Juan C.; Van Rompay, Koen.

In: Journal of Virology, Vol. 80, No. 18, 01.09.2006, p. 9217-9225.

Research output: Contribution to journalArticle

Forthal, Donald N. ; Landucci, Gary ; Cole, Kelly Stefano ; Marthas, Marta ; Becerra, Juan C. ; Van Rompay, Koen. / Rhesus macaque polyclonal and monoclonal antibodies inhibit simian immunodeficiency virus in the presence of human or autologous rhesus effector cells. In: Journal of Virology. 2006 ; Vol. 80, No. 18. pp. 9217-9225.
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