Rhesus macaque blastocysts resulting from intracytoplasmic sperm injection of vacuum-dried spermatozoa

Stuart A Meyers, Ming Wen Li, Allen C. Enders, James W. Overstreet

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Sperm desiccation is an attractive approach for sperm preservation. In this study, we examined the feasibility and efficiency of intracytoplasmic sperm injection using vacuum-dried rhesus macaque sperm in CZB medium supplemented with 10% fetal bovine serum. Methods: A total of 109 MII oocytes were injected with 69 fresh ejaculated sperm and 40 vacuum-dried sperm. Results: Cleavage occurred in 97% of oocytes injected with fresh, motile sperm and in 88% of oocytes injected with vacuum-dried sperm. Of the cleaved oocytes, 68% fresh sperm-injected oocytes and 74% of dried sperm-injected oocytes developed to the compact morula stage. Blastocyst development was comparable between fresh-injected (16%) and vacuum-dried-injected (17%) oocytes. Differences between treatment groups were not significant. Transmission electron microscopic observation of the blastocysts indicated no detectable differences between fresh sperm and dried sperm-derived embryos. Conclusions: We conclude that vacuum-dried rhesus macaque sperm are capable of inducing fertilization and development of pre-implantation embryos when sperm were dried under vacuum and microinjected into normal viable oocytes.

Original languageEnglish (US)
Pages (from-to)310-317
Number of pages8
JournalJournal of Medical Primatology
Volume38
Issue number5
DOIs
StatePublished - 2009

Fingerprint

intracytoplasmic sperm injection
Intracytoplasmic Sperm Injections
Blastocyst
Vacuum
Macaca mulatta
blastocyst
Spermatozoa
spermatozoa
Oocytes
oocytes
Semen Preservation
Morula
Desiccation
embryo implantation
morula
fetal bovine serum
Fertilization
fertilization (reproduction)
Embryonic Development

Keywords

  • Dried sperm storage
  • Early embryonic development
  • Intracytoplasmic sperm injection
  • Monkey embryos

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Rhesus macaque blastocysts resulting from intracytoplasmic sperm injection of vacuum-dried spermatozoa. / Meyers, Stuart A; Li, Ming Wen; Enders, Allen C.; Overstreet, James W.

In: Journal of Medical Primatology, Vol. 38, No. 5, 2009, p. 310-317.

Research output: Contribution to journalArticle

Meyers, Stuart A ; Li, Ming Wen ; Enders, Allen C. ; Overstreet, James W. / Rhesus macaque blastocysts resulting from intracytoplasmic sperm injection of vacuum-dried spermatozoa. In: Journal of Medical Primatology. 2009 ; Vol. 38, No. 5. pp. 310-317.
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