Rhesus macaque blastocysts resulting from intracytoplasmic sperm injection of vacuum-dried spermatozoa

Stuart A Meyers, Ming Wen Li, Allen C. Enders, James W. Overstreet

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Background: Sperm desiccation is an attractive approach for sperm preservation. In this study, we examined the feasibility and efficiency of intracytoplasmic sperm injection using vacuum-dried rhesus macaque sperm in CZB medium supplemented with 10% fetal bovine serum. Methods: A total of 109 MII oocytes were injected with 69 fresh ejaculated sperm and 40 vacuum-dried sperm. Results: Cleavage occurred in 97% of oocytes injected with fresh, motile sperm and in 88% of oocytes injected with vacuum-dried sperm. Of the cleaved oocytes, 68% fresh sperm-injected oocytes and 74% of dried sperm-injected oocytes developed to the compact morula stage. Blastocyst development was comparable between fresh-injected (16%) and vacuum-dried-injected (17%) oocytes. Differences between treatment groups were not significant. Transmission electron microscopic observation of the blastocysts indicated no detectable differences between fresh sperm and dried sperm-derived embryos. Conclusions: We conclude that vacuum-dried rhesus macaque sperm are capable of inducing fertilization and development of pre-implantation embryos when sperm were dried under vacuum and microinjected into normal viable oocytes.

Original languageEnglish (US)
Pages (from-to)310-317
Number of pages8
JournalJournal of Medical Primatology
Volume38
Issue number5
DOIs
StatePublished - 2009

Keywords

  • Dried sperm storage
  • Early embryonic development
  • Intracytoplasmic sperm injection
  • Monkey embryos

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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