Rhein inhibits TPA-induced activator protein-1 activation and cell transformation by blocking the JNK-dependent pathway.

Shigang Lin, Jian-Jian Li, Makoto Fujii, De Xing Hou

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Rhein (4,5-Dihydroxyanthraquinone-2-carboxylic acid), a constituent enriched in the rhizome of rhubarb (R. palmatum L. or R. tanguticum Maxim), is a traditional Chinese herb used as a laxative and stomachic drug. In the present study, we investigated the anti-carcinogenesis of rhein by using mouse epidermal cell JB6 line, an in vitro model for elucidating the molecular mechanisms of cancer chemopreventive agents. Rhein is shown to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation and activator protein-1 (AP-1) activation in a dose-dependent manner. Signal cascade analysis revealed that rhein inhibits the phosphorylation and abundance of c-Jun protein, c-Jun NH2-terminal kinase (JNK) phosphorylation, but does not inhibit the phosphorylation of extracellular signal-regulated protein kinase (ERK) and p38 kinase. Thus, these results provide the first evidence suggesting that rhein inhibits AP-1 activity and cell transformation through the inhibition of a JNK-dependent, ERK- and p38-independent molecular mechanism.

Original languageEnglish (US)
Pages (from-to)829-833
Number of pages5
JournalInternational Journal of Oncology
Volume22
Issue number4
StatePublished - Apr 2003
Externally publishedYes

Fingerprint

JNK Mitogen-Activated Protein Kinases
Transcription Factor AP-1
Tetradecanoylphorbol Acetate
Phosphorylation
Proto-Oncogene Proteins c-jun
Rheum
Laxatives
Rhizome
Molecular Models
Extracellular Signal-Regulated MAP Kinases
Protein Kinases
rhein
Carcinogenesis
Phosphotransferases
Cell Line
Pharmaceutical Preparations
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Rhein inhibits TPA-induced activator protein-1 activation and cell transformation by blocking the JNK-dependent pathway. / Lin, Shigang; Li, Jian-Jian; Fujii, Makoto; Hou, De Xing.

In: International Journal of Oncology, Vol. 22, No. 4, 04.2003, p. 829-833.

Research output: Contribution to journalArticle

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AU - Hou, De Xing

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