Rhabditid nematode-associated ophthalmitis and meningoencephalomyelitis in captive Asian horned frogs (Megophrys montana)

Denise Imai, Steven A. Nadler, Deena Brenner, Taryn A. Donovan, Allan P. Pessier

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Between 2006 and 2008, 4 captive Asian horned frogs (Megophrys montana) were diagnosed with ocular and neurologic disease associated with rhabditid nematodiasis. Mortality, either spontaneous or by humane euthanasia, was high (3/4, 75%). Gross and histologic findings included varying degrees of ulcerative keratitis, histiocytic uveitis and retinitis, meningoencephalomyelitis, and epidermal chromatophore (iridophore) hyperplasia with intralesional nematodes. Entry into the host was presumed to be by direct invasion of the skin and the cornea with migration through the optic nerve to the brain and spinal cord. One frog was diagnosed with rhabditid nematodiasis antemortem, and clinical signs and lesions in the frog did not progress after unilateral enucleation and anthelminthic treatment were completed. Gross and tissue morphology of the nematodes were consistent with the order Rhabditida. DNA was extracted separately from 2 individual nematodes that were isolated from frozen and ethanol-preserved eye and brain tissue. These DNA templates were used for polymerase chain reaction amplification and sequencing of nuclear 28S large subunit (LSU) and internal transcribed spacer (ITS) ribosomal DNA regions. Comparison of the LSU and ITS sequences to those deposited in GenBank revealed an exact match for Caenorhabditis elegans.

Original languageEnglish (US)
Pages (from-to)568-573
Number of pages6
JournalJournal of Veterinary Diagnostic Investigation
Volume21
Issue number4
DOIs
StatePublished - Jan 1 2009

Fingerprint

meningoencephalitis
Anura
frogs
Nematoda
internal transcribed spacers
Rhabditida
Ribosomal Spacer DNA
Chromatophores
eye diseases
brain
Retinitis
Corneal Ulcer
chromatophores
keratitis
Euthanasia
nervous system diseases
Eye Diseases
DNA
Uveitis
Nucleic Acid Databases

Keywords

  • Asian horned frogs
  • Caenorhabditis elegans
  • Megophrys
  • Verminous ophthalmitis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Rhabditid nematode-associated ophthalmitis and meningoencephalomyelitis in captive Asian horned frogs (Megophrys montana). / Imai, Denise; Nadler, Steven A.; Brenner, Deena; Donovan, Taryn A.; Pessier, Allan P.

In: Journal of Veterinary Diagnostic Investigation, Vol. 21, No. 4, 01.01.2009, p. 568-573.

Research output: Contribution to journalArticle

Imai, Denise ; Nadler, Steven A. ; Brenner, Deena ; Donovan, Taryn A. ; Pessier, Allan P. / Rhabditid nematode-associated ophthalmitis and meningoencephalomyelitis in captive Asian horned frogs (Megophrys montana). In: Journal of Veterinary Diagnostic Investigation. 2009 ; Vol. 21, No. 4. pp. 568-573.
@article{210bffb280ab49aba9cc39e3220e818d,
title = "Rhabditid nematode-associated ophthalmitis and meningoencephalomyelitis in captive Asian horned frogs (Megophrys montana)",
abstract = "Between 2006 and 2008, 4 captive Asian horned frogs (Megophrys montana) were diagnosed with ocular and neurologic disease associated with rhabditid nematodiasis. Mortality, either spontaneous or by humane euthanasia, was high (3/4, 75{\%}). Gross and histologic findings included varying degrees of ulcerative keratitis, histiocytic uveitis and retinitis, meningoencephalomyelitis, and epidermal chromatophore (iridophore) hyperplasia with intralesional nematodes. Entry into the host was presumed to be by direct invasion of the skin and the cornea with migration through the optic nerve to the brain and spinal cord. One frog was diagnosed with rhabditid nematodiasis antemortem, and clinical signs and lesions in the frog did not progress after unilateral enucleation and anthelminthic treatment were completed. Gross and tissue morphology of the nematodes were consistent with the order Rhabditida. DNA was extracted separately from 2 individual nematodes that were isolated from frozen and ethanol-preserved eye and brain tissue. These DNA templates were used for polymerase chain reaction amplification and sequencing of nuclear 28S large subunit (LSU) and internal transcribed spacer (ITS) ribosomal DNA regions. Comparison of the LSU and ITS sequences to those deposited in GenBank revealed an exact match for Caenorhabditis elegans.",
keywords = "Asian horned frogs, Caenorhabditis elegans, Megophrys, Verminous ophthalmitis",
author = "Denise Imai and Nadler, {Steven A.} and Deena Brenner and Donovan, {Taryn A.} and Pessier, {Allan P.}",
year = "2009",
month = "1",
day = "1",
doi = "10.1177/104063870902100427",
language = "English (US)",
volume = "21",
pages = "568--573",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "4",

}

TY - JOUR

T1 - Rhabditid nematode-associated ophthalmitis and meningoencephalomyelitis in captive Asian horned frogs (Megophrys montana)

AU - Imai, Denise

AU - Nadler, Steven A.

AU - Brenner, Deena

AU - Donovan, Taryn A.

AU - Pessier, Allan P.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Between 2006 and 2008, 4 captive Asian horned frogs (Megophrys montana) were diagnosed with ocular and neurologic disease associated with rhabditid nematodiasis. Mortality, either spontaneous or by humane euthanasia, was high (3/4, 75%). Gross and histologic findings included varying degrees of ulcerative keratitis, histiocytic uveitis and retinitis, meningoencephalomyelitis, and epidermal chromatophore (iridophore) hyperplasia with intralesional nematodes. Entry into the host was presumed to be by direct invasion of the skin and the cornea with migration through the optic nerve to the brain and spinal cord. One frog was diagnosed with rhabditid nematodiasis antemortem, and clinical signs and lesions in the frog did not progress after unilateral enucleation and anthelminthic treatment were completed. Gross and tissue morphology of the nematodes were consistent with the order Rhabditida. DNA was extracted separately from 2 individual nematodes that were isolated from frozen and ethanol-preserved eye and brain tissue. These DNA templates were used for polymerase chain reaction amplification and sequencing of nuclear 28S large subunit (LSU) and internal transcribed spacer (ITS) ribosomal DNA regions. Comparison of the LSU and ITS sequences to those deposited in GenBank revealed an exact match for Caenorhabditis elegans.

AB - Between 2006 and 2008, 4 captive Asian horned frogs (Megophrys montana) were diagnosed with ocular and neurologic disease associated with rhabditid nematodiasis. Mortality, either spontaneous or by humane euthanasia, was high (3/4, 75%). Gross and histologic findings included varying degrees of ulcerative keratitis, histiocytic uveitis and retinitis, meningoencephalomyelitis, and epidermal chromatophore (iridophore) hyperplasia with intralesional nematodes. Entry into the host was presumed to be by direct invasion of the skin and the cornea with migration through the optic nerve to the brain and spinal cord. One frog was diagnosed with rhabditid nematodiasis antemortem, and clinical signs and lesions in the frog did not progress after unilateral enucleation and anthelminthic treatment were completed. Gross and tissue morphology of the nematodes were consistent with the order Rhabditida. DNA was extracted separately from 2 individual nematodes that were isolated from frozen and ethanol-preserved eye and brain tissue. These DNA templates were used for polymerase chain reaction amplification and sequencing of nuclear 28S large subunit (LSU) and internal transcribed spacer (ITS) ribosomal DNA regions. Comparison of the LSU and ITS sequences to those deposited in GenBank revealed an exact match for Caenorhabditis elegans.

KW - Asian horned frogs

KW - Caenorhabditis elegans

KW - Megophrys

KW - Verminous ophthalmitis

UR - http://www.scopus.com/inward/record.url?scp=67849128695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67849128695&partnerID=8YFLogxK

U2 - 10.1177/104063870902100427

DO - 10.1177/104063870902100427

M3 - Article

C2 - 19564514

AN - SCOPUS:67849128695

VL - 21

SP - 568

EP - 573

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 4

ER -